产品名称
来自西非单叶豆（加纳籽）的凝集素, Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder
biological source
Bandieraea simplicifolia
conjugate
biotin conjugate
form
lyophilized powder
potency
<50 μg per mL agglutination activity (using human blood group B erythrocytes)
composition
Protein, ~95% E1%/280
extent of labeling
3-6 mol biotin per mol protein
storage temp.
−20°C
Quality Level
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Analysis Note
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
Application
来自西非单叶豆（加纳籽）的凝集素已用于免疫组化和免疫荧光染色。
Biochem/physiol Actions
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
来自西非单叶豆（加纳籽）的凝集素可凝集人A和B血型细胞。它对αGalNAc-和αGal具有特异性，可作为试剂用于检测生物材料中的αGal表位。凝集素（来自西非单叶豆）可用作异种反应性抗原(αGal1−3Gal)和癌症的标志物。凝集素在受精、胚胎发生、炎症、癌细胞转移和宿主-寄生物识别中起重要作用。
General description
来自西非单叶豆（加纳籽）的凝集素具有A和B两种亚基，分别识别αGalNAc和αGal端基。凝集素从豆科灌木加纳籽(Griffonia simplicifolia)的种子中提取，是糖类结合蛋白。
Other Notes
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
Physical form
含柠檬酸盐缓冲盐和氯化钙
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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