生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
MTA30 2G10/3, monoclonal
表单
buffered aqueous solution
分子量
antigen ~59 kDa
种属反应性
human, monkey
技术
immunoprecipitation (IP): suitable
western blot: 0.5-1 μg/mL using HEK-293T total cell extract
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MUS81(80198)
一般描述
Monoclonal Anti-Mus81 (mouse IgG2a isotype) is derived from the hybridoma MTA30 2G10/3 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with Mus81 protein. Crossover junction endonuclease Mus81 protein was identified by screening a human cerebellum cDNA library with a probe from the yeast Mus81. The human Mus81 has 551 amino acids with a molecular mass of 59 kDa. The highest sequence similarity between different species is around the VERKX3D motif. This motif is conserved in the endonuclease family (XPF) family of nucleases. The protein has helix-hairpin-helix DNA-binding domains in the N- and C-termini of the protein that are also conserved.
免疫原
Mus81 protein.
应用
Anti-Mus81 antibody, Mouse monoclonal has been used in immunofluorescence, immunoblotting and immunoprecipitation.
生化/生理作用
Crossover junction endonuclease Mus81 is elevated in cells exposed to agents that block DNA replication. Knockout mice that are homozygous Mus81-/-, or their heterozygous cohorts Mus81-/+, are susceptible to spontaneous chromosomal damage and cancer predisposition suggesting that this protein is important for genomic integrity and tumor suppression
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Antonio Porro et al.
Nature communications, 8(1), 1073-1073 (2017-10-21)
Interstrand cross-link (ICL) hypersensitivity is a characteristic trait of Fanconi anemia (FA). Although FANCD2-associated nuclease 1 (FAN1) contributes to ICL repair, FAN1 mutations predispose to karyomegalic interstitial nephritis (KIN) and cancer rather than to FA. Thus, the biological role of
Regulation of multiple DNA repair pathways by the Fanconi anemia protein SLX4
Kim Y, et al.
Blood, 121(1), 54-63 (2013)
Nadeem Shaikh et al.
Genome biology, 23(1), 223-223 (2022-10-22)
A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induced replication stress induces breakages at common fragile sites, but the exact
Oncogenes induce genotoxic stress by mitotic processing of unusual replication intermediates
Neelsen KJ, et al.
The Journal of Cell Biology, 200(6), 699-708 (2013)
Human Mus81-associated endonuclease cleaves Holliday junctions in vitro
Chen XB, et al.
Molecular Cell, 8(5), 1117-1127 (2001)
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