生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~80 kDa
种属反应性
human
浓度
~1 mg/mL
技术
indirect immunofluorescence: 1-2 μg/mL using HeK 293T cells transfected with MBD1 (isoform 3), fixed with paraformaldehyde-triton
microarray: suitable
western blot: 0.25-0.5 μg/mL using extracts of HEK 293T cells transfected with MBD1 (isoform 3)
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MBD1(4152)
一般描述
MBD1 gene is mapped to human chromosome 18q21.1.
Methyl-CpG binding domain protein 1 (MBD1) exists as five isoforms.
免疫原
synthetic peptide corresponding to amino acids 591-605 of human MBD1, conjugated to KLH via an N-terminal added lysine residue. This sequence is found in MBD1 isoforms.
应用
Anti-MBD1 antibody produced in rabbit may be used for immunofluorescence and immunoblotting.
生化/生理作用
Anti MBD1 recognizes recombinant human MBD1 (~80 kDa).
MBD1 can act as a transcriptional repressor. It mediates transcriptional repression by recruiting heterochromatin-binding protein (HP1), the histone methyl transferase Suv39h1 and histone deacetylase using the methyl binding domain (MBD). The transcriptional repression domain (TRD) of MBD1 mediates its interaction with MBD1-containing chromatin-associated factor (MCAF-1).
Methyl-CpG binding domain protein 1 (MBD1) aids pancreatic cancer cells in drug resistance and contributes to disease progression. It gets recruited to those sites where DNA damage has occurred and the protein has also shown to be involved in suppression of gene transcription. MBD1 acts as a link between methylation of DNA and repression of transcription.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
制备说明
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
新产品
Holly N Cukier et al.
Neurogenetics, 11(3), 291-303 (2009-11-19)
Misregulation of the methyl-CpG-binding protein 2 (MECP2) gene has been found to cause a myriad of neurological disorders including autism, mental retardation, seizures, learning disabilities, and Rett syndrome. We hypothesized that mutations in other members of the methyl-CpG-binding domain (MBD)
Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.
Liu C
BMC Cancer, 8, 121-121 (2008)
Silencing of MBD1 reverses pancreatic cancer therapy resistance through inhibition of DNA damage repair.
Xu J
International Journal of Oncology, 42(6), 2046-2052 (2013)
Takaya Ichimura et al.
The Journal of biological chemistry, 280(14), 13928-13935 (2005-02-05)
DNA methylation cooperates with methylation at lysine 9 of histone H3 (H3-K9), a modified histone molecule that is targeted by heterochromatin protein 1, to form a transcriptionally silent chromatin. Methyl CpG-binding protein MBD1 recognizes methylated CpG dinucleotide and recruits H3-K9
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