form
lyophilized powder
specific activity
≥50 units/mg solid
storage temp.
−20°C
General description
Malic dehydrogenase from Thermus flavus is stable and active at 90 ° C.
Application
Malic dehydrogenase has been used in a study to assess electron transport chain activity in mitochondria from human skeletal muscle. It has also been used in a study to investigate activities of enzymes and ammonia in serum of rats with fluoride hyperglycemia.
Physical form
Contains dextran
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
C A Kelly et al.
Biochemistry, 32(15), 3913-3922 (1993-04-20)
A binary complex of malate dehydrogenase from the thermophilic bacterium Thermus flavus (tMDH) with NADH has been crystallized from poly(ethylene glycol) 3500, pH 8.5, yielding diffraction-quality crystals in space group P2(1)2(1)2(1). The structure was solved at 1.9-A resolution using molecular
Ewa Grucka-Mamczar et al.
Annales Academiae Medicae Stetinensis, 50 Suppl 1, 36-41 (2006-08-09)
The study was done in 30 one-month-old Wistar FL rats divided into one control and two study groups of ten animals each. Hyperglycemia was induced with sodium fluoride in water at a concentration of 50 or 100 mg/L during four
G Ferreira et al.
Journal of dairy science, 90(3), 1460-1466 (2007-02-14)
Biotin is a cofactor of the gluconeogenic enzymes pyruvate carboxylase (PC) and propionyl-coenzyme A carboxylase (PCC). We hypothesized that biotin supplementation increases the activity and gene expression of PC and PCC and the gene expression of phosphoenol-pyruvate carboxykinase (PEPCK) in
Vladimir B Ritov et al.
Analytical biochemistry, 333(1), 27-38 (2004-09-08)
This study addresses an application of pyridine nucleotide enzymatic analyses to evaluate the activity of the mitochondrial electron transport chain (reduced nicotinamide adenine dinucleotide (NADH) oxidase) and Complexes I and II in samples of human muscle as small as approximately
Clelia Muccio et al.
The protein journal, 31(8), 667-673 (2012-09-12)
A novel malate dehydrogenase (MDH; EC 3.1.1.1.37), hereafter MDHCs, from Ceratonia siliqua seeds, commonly known as Carob tree, was purified by using ammonium sulphate precipitation, ion exchange chromatography on SteamLine SP and gel-filtration. The molecular mass of the native protein
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