M7278
Eagle最低必需培养基
HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
MEM
Product Name
Eagle最低必需培养基, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
pH值（酸碱度）
>7.2
组分
L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes
运输
ambient
储存温度
2-8°C
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一般描述
EMEM培养基由糖、无机盐、水溶性维生素和必需氨基酸组成，广泛用于许多细胞培养基研究的培养基配方。
应用
EMEM培养基用于培养原始卵泡和卵巢组织、人体皮肤成纤维细胞、人结肠上皮细胞系 LS174T 和海马组织切片等。
制备说明
添加 0.292g/L L-谷氨酰胺。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
Samuel Wilson Failor et al.
Neural development, 13(1), 4-4 (2018-03-27)
Activity in neurons drives afferent competition that is critical for the refinement of nascent neural circuits. In ferrets, when an eye is lost in early development, surviving retinogeniculate afferents from the spared eye spread across the thalamus in a manner
Ayako Suzuki et al.
Scientific reports, 9(1), 19529-19529 (2019-12-22)
To better understand the disruptions of transcriptional regulations and gene expression in lung cancers, we constructed a multi-omics catalogue of the responses of lung cancer cells to a series of chemical compounds. We generated and analyzed 3,240 RNA-seq and 3,393
Johannes Vierock et al.
Nature communications, 12(1), 4527-4527 (2021-07-28)
Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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