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Merck
CN

M7278

Sigma-Aldrich

Eagle最低必需培养基

HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

product name

Eagle最低必需培养基, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

>7.2

组分

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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一般描述

EMEM培养基由糖、无机盐、水溶性维生素和必需氨基酸组成,广泛用于许多细胞培养基研究的培养基配方。

应用

EMEM培养基用于培养原始卵泡和卵巢组织、人体皮肤成纤维细胞、人结肠上皮细胞系 LS174T 和海马组织切片等。

重悬

添加 0.292g/L L-谷氨酰胺。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Samuel Wilson Failor et al.
Neural development, 13(1), 4-4 (2018-03-27)
Activity in neurons drives afferent competition that is critical for the refinement of nascent neural circuits. In ferrets, when an eye is lost in early development, surviving retinogeniculate afferents from the spared eye spread across the thalamus in a manner
Rodrigo Lerchundi et al.
Frontiers in cellular neuroscience, 14, 80-80 (2020-05-07)
Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro
Shafagh A Waters et al.
The EMBO journal, 36(3), 374-387 (2016-11-12)
RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)

商品

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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