用途
sufficient for 100 colorimetric or fluorometric tests
检测方法
colorimetric
fluorometric
相关疾病
cancer
储存温度
−20°C
基因信息
human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164)
mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908)
rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)
一般描述
磷脂酶 D (PLD) 催化甘油磷脂的磷酸二酯键水解生成磷脂酸和胆碱。它被广泛表达，参与免疫功能和癌症抑制。PLD 表达异常与人类癌症相关，如乳腺癌、肾癌、胃癌和结直肠癌。
应用
适于检测生物样品中的磷脂酶 D 的活性。 本试剂盒可用于研究各种化合物对磷脂酶 D 活性的影响。
适用于磷脂酶 D 活性的定量测定及药物对磷脂酶 D 代谢影响的评价。
采用磷脂酶 D (PLD) 活性检测试剂盒测定了小鼠肝脏和肝脏溶酶体中 PLD 活性。
生化/生理作用
在本试验中，PLD 将磷脂酰胆碱水解为胆碱，使用胆碱氧化酶进行测定，得到比色 (570 nm)/荧光（λex = 530/λem = 585 nm）乘积，与样品中 PLD 活性成正比。单位定义：在试验条件下 (pH 7.4)，1 个单位的 PLD 每分钟催化 1 mmol 胆碱的形成。
特点和优势
兼容高通量的操作系统。
警示用语：
Danger
危险声明
危险分类
Aquatic Chronic 3 - Resp. Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
Phospholipase D.
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