3′ to 5′ Exonuclease Activity Assay (Fluorometric)

Exonucleases remove a single nucleotide or an oligonucleotide at the 5′ or 3′ end. Exonuclease, such as Three Prime Repair Exonuclease 1 (TREX1), plays an important role in proofreading of DNA amplification and removal of damaged DNA. Deficiency of TREX1 enzyme has been shown to trigger the autoimmune disease Aicardia-Goutieres syndrome. TREX1 expression has been reported to correlate with cervical cancer cell growth and disease progression. In addition, 3′ exonucleases are often used as tools in molecular biology such as site-directed mutagenesis and the generation of single stranded DNA probe

The kit is suitable for the measurement of 3′ to 5′ exonuclease activity in biological samples (mammalian and bacterial cell culture and animal tissue) and as quality control of purified 3′ to 5′ exonuclease.

In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (λ_Ex = 304 nm/λ_Em = 369 nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 μU of exonuclease activity.

3′ to 5′ Exonuclease Activity Assay:

• Compatible with high-throughput handling systems.
• Sensitive, detecting as low as 0.2 μU of exonuclease activity.