用途
vial sufficient for 50 reactions
运输
dry ice
储存温度
−70°C
应用
A cell-free transcription system that produces accurate transcripts with RNA polymeraseII. Reaction will incorporate 50-150fmol nucleotides into a 400-nucleotide transcript from Ad2ML (adenovirus major late promoter) in 60min at 30°C.
生化/生理作用
The system carries out basal transcription, dependent on recognition of the TATA element, as well as transcription which is regulated by interaction of transcription factors with upstream promoter elements, e.g., USF, Oct-1, Sp-1, progesterone receptor.
Depleting the extract of individual transcription factors produces a system for assaying purified factors (TATA binding protein, Oct-1, etc.) by complementation. The extract is also a source of transcriptional regulators and RNA processing proteins.
Depleting the extract of individual transcription factors produces a system for assaying purified factors (TATA binding protein, Oct-1, etc.) by complementation. The extract is also a source of transcriptional regulators and RNA processing proteins.
包装
Supplied in 20 mM HEPES, pH 9.0, containing 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, and 20% glycerol.
分析说明
The extract has been treated with PMSF to inhibit proteases
法规信息
新产品
此项目有
T M Kristie et al.
The EMBO journal, 8(13), 4229-4238 (1989-12-20)
The herpes simplex virus transactivator, alpha TIF, stimulates transcription of the alpha/immediate early genes via a cis-acting site containing an octamer element and a conserved flanking sequence. The alpha TIF protein, produced in a baculovirus expression system, nucleates the formation
Regulation of in vitro transcription by progesterone receptor. Characterization and kinetic studies.
M K Bagchi et al.
The Journal of biological chemistry, 265(9), 5129-5134 (1990-03-25)
We have devised an in vitro assay system to study the transcriptional activity of native chicken progesterone receptor (cPR). Purified cPR added to cell-free extracts from HeLa cell nuclei stimulates accurate transcription from a promoter driven by two progesterone response
Sawadogo, M., et al.
The Journal of Biological Chemistry, 263, 11985-11985 (1985)
Briggs, M.R., et al.
Science, 243, 47-47 (1986)
R W Carthew et al.
Cell, 43(2 Pt 1), 439-448 (1985-12-01)
A gel electrophoresis DNA binding assay has been used to identify proteins in HeLa cell extracts that specifically bind to the major late promoter of adenovirus. A major late promoter transcription factor MLTF has been detected as a discrete protein-DNA
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