N2635
Nucleoside 5′-Diphosphate Kinase from bovine liver
buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)
别名:
NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
登录 查看组织和合同定价。
选择规格
变更视图
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥1,000 units/mg protein (biuret)
Biological source:
bovine liver
biological source
bovine liver
Quality Segment
form
buffered aqueous glycerol solution
specific activity
≥1,000 units/mg protein (biuret)
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
foreign activity
lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
storage temp.
2-8°C
General description
Research area: Cell Signaling
Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.
Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.
Application
Nucleoside 5′-Diphosphate Kinase has been used:
- nucleoside diphosphate kinase (NDPK) assays
- coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
- non-metastatic protein 23 (NM23) growth stimulation assay
- in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells
- in a study to investigate protein synthesis in rabbit reticulocytes
Biochem/physiol Actions
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.
Other Notes
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable