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Merck
CN

N5759

4-硝基苯基 β- D -纤维二糖

chromogenic, ≥98% (TLC), powder

别名:

p -硝基苯基 β- D -纤维二糖

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关于此项目

经验公式(希尔记法):
C18H25NO13
化学文摘社编号:
分子量:
463.39
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
100234
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产品名称

4-硝基苯基 β- D -纤维二糖, ≥98% (TLC)

Quality Level

assay

≥98% (TLC)

form

powder

solubility

water: 49.00-51.00 mg/mL

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1

InChI key

IAYJZWFYUSNIPN-KFRZSCGFSA-N

Application

4-硝基苯基β-D-吡喃木糖苷已用作底物,以测定β-D-纤维二糖苷酶或纤维二糖水解酶(胞外纤维素酶)在微生物群落中的活性。它还可用作底物来研究内切葡聚糖酶J30的活性。

Biochem/physiol Actions

4-硝基苯基 β-D-吡喃木糖苷是一种纤维三糖类似物,和常用于检测纤维素酶活性的显色底物。外切葡聚糖酶、内切葡聚糖酶和β -葡糖苷酶可水解4-硝基苯基 β-D-吡喃木糖苷,以形成p-硝基酚(PNP)。


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

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R M Gardner et al.
Journal of bacteriology, 169(10), 4581-4588 (1987-10-01)
An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter
K Igarashi et al.
European journal of biochemistry, 253(1), 101-106 (1998-05-13)
The interaction of cellobiose dehydrogenase (CDH) with cellobiohydrolase I (CBH I) in cellulose-grown cultures of Phanerochaete chrysosporium was investigated to clarify the role of CDH in cellulose degradation. Decomposition of bacterial microcrystalline cellulose by CBH I was enhanced significantly in
S J Han et al.
The Journal of biological chemistry, 270(43), 26012-26019 (1995-10-27)
Bacillus sp. D04 secreted a bifunctional cellulase that had a molecular weight of 35,000. This cellulase degraded Cm-cellulose, cellotetraose, cellopentaose, p-nitrophenyl-beta-D-cellobioside, and avicel PH101. Based on the high performance liquid chromatography analysis of the degradation products, this cellulase randomly cleaved



全球贸易项目编号

货号GTIN
N5759-100MG04061834117193
N5759-500MG04061838446411