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Merck
CN

N5759

4-硝基苯基 β- D -纤维二糖

chromogenic, ≥98% (TLC), powder

别名:

p -硝基苯基 β- D -纤维二糖

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关于此项目

经验公式(希尔记法):
C18H25NO13
化学文摘社编号:
分子量:
463.39
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
100234
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产品名称

4-硝基苯基 β- D -纤维二糖, ≥98% (TLC)

Quality Level

assay

≥98% (TLC)

form

powder

solubility

water: 49.00-51.00 mg/mL

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1

InChI key

IAYJZWFYUSNIPN-KFRZSCGFSA-N

Application

4-硝基苯基β-D-吡喃木糖苷已用作底物,以测定β-D-纤维二糖苷酶或纤维二糖水解酶(胞外纤维素酶)在微生物群落中的活性。它还可用作底物来研究内切葡聚糖酶J30的活性。

Biochem/physiol Actions

4-硝基苯基 β-D-吡喃木糖苷是一种纤维三糖类似物,和常用于检测纤维素酶活性的显色底物。外切葡聚糖酶、内切葡聚糖酶和β -葡糖苷酶可水解4-硝基苯基 β-D-吡喃木糖苷,以形成p-硝基酚(PNP)。


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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Daniel J Coleman et al.
Analytical biochemistry, 371(2), 146-153 (2007-10-12)
A simple and reliable continuous assay procedure for measurement of cellulase activity from several species using the new substrate resorufin-beta-D-cellobioside (Res-CB) has been developed. The product of enzyme reaction, resorufin, exhibits fluorescence emission at 585 nm with excitation at 571
Thomas L Ellinghaus et al.
Acta crystallographica. Section D, Structural biology, 74(Pt 7), 702-710 (2018-07-04)
The development of robust enzymes, in particular cellulases, is a key step in the success of biological routes to `second-generation' biofuels. The typical sources of the enzymes used to degrade biomass include mesophilic and thermophilic organisms. The endoglucanase J30 from
K Ohmiya et al.
Journal of bacteriology, 161(1), 432-434 (1985-01-01)
An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was



全球贸易项目编号

货号GTIN
N5759-100MG04061834117193
N5759-500MG04061838446411