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Merck
CN

N7271

Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

别名:

受体破坏酶, 唾液酸酶, 神经氨酸苷酶

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关于此项目

CAS Number:
9001-67-6
EC 号:
3.2.1.18 (BRENDA, IUBMB)
MDL编号:
MFCD01092203
UNSPSC代码:
12352204
NACRES:
NA.32

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生物来源

Streptococcus pneumoniae

质量水平

100

表单

buffered aqueous solution

浓度

≥5 units/mL

异质活性

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

运输

wet ice

储存温度

2-8°C

基因信息

Streptococcus pneumoniae R6 ... nanB(933504)

相关类别

生化/生理作用

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

包装

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

外形

Solution in 50 mM sodium phosphate, pH 7.5

制备说明

Expressed in glycosidase-free hosts.

其他说明

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCV1402
MKCC6247
MKCB0382V
MKCB0222V
MKBT5173V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

  6. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

  7. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

  8. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Alana L Woodward et al.
Veterinary microbiology, 169(3-4), 113-127 (2014-02-01)
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during 2010-2012, including in vaccinated animals, highlighting the importance of surveillance and virus characterisation. Virus isolates
Chinh Tran-To Su et al.
BMC bioinformatics, 14 Suppl 16, S7-S7 (2014-02-26)
Since late March 2013, there has been another global health concern with a sudden wave of flu infections by a novel strain of avian influenza A (H7N9) virus in China. To-date, there have been more than 100 infections with 23
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
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