recombinant
expressed in E. coli
Quality Segment
form
buffered aqueous solution
specific activity
≥135 units/mg protein
mol wt
88 kDa, 95 kDa
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
从复合寡糖中释放α(2→3)、α(2→6)、α(2→8)和 α(2→9)-连接的N-乙酰神经氨酸。
Packaging
本品为5倍浓缩的反应缓冲液(250 mM磷酸钠,pH 6.0)。
Physical form
溶于20mM Tris-HCl (pH 7.5)和20 mM NaCl的溶液中。
Preparation Note
表达于无糖苷酶的宿主中。
Other Notes
一个酶活性单位是指在pH 5.0、37℃下,每分钟水解1 μmole的4-甲基伞形酮α-D -N-乙酰神经氨酸苷所需的酶量。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)