N9914
Polynucleotide phosphorylase from Synechocystis sp.
recombinant, expressed in E. coli
别名:
PNPase, Polyribonucleotide Nucleotidyltransferase
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关于此项目
Specific activity:
≥500 units/mg protein
Assay:
90% (SDS-PAGE)
Biological source:
bacterial (Synechocystis sp.)
Recombinant:
expressed in E. coli
biological source
bacterial (Synechocystis sp.)
Quality Segment
recombinant
expressed in E. coli
description
Histidine tagged
assay
90% (SDS-PAGE)
form
solution
specific activity
≥500 units/mg protein
mol wt
85 kDa
technique(s)
cell based assay: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
General description
Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.
Application
Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.
Biochem/physiol Actions
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.
Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.
Other Notes
One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable