capacity
≤20 μg binding capacity
Application
The GenElute Gel Extraction Kit is designed for the rapid purification of DNA of 100 bp to 10 kb in length, from standard or low-melting agarose gels in TAE or TBE buffer. A typical recovery is 50-55% with recoveries as high as 80%. The GenElute Gel Extraction Kit combines silica based-membrane technology and the convenience of a spin column format to extract and purify DNA fragments of interest from agarose gels.
Biochem/physiol Actions
DNA fragments of interest are extracted from slices of an agarose gel by solubilizing the gel. The Gel Solubilization Solution contains a pH indicator, which allows the gel slice to be visualized easily as well as indicating whether the pH is optimal for binding. DNA fragments of interest are selectively adsorbed onto the silica membrane and contaminants are then removed by a simple spin-wash step. Finally, the bound DNA is eluted in water or Tris buffer. The eluted DNA is suitable for a variety of downstream applications including automated sequencing, PCR, restriction digestion, cloning and labeling. Columns are designed to removed dsDNA between 100 bp and 10 kb.
Features and Benefits
- DNA fragments purified in as little as 15 minutes
- Up to 80% recovery of DNA fragments
- Purified DNA is suitable for all common downstream applications
- No toxic organic solvents or precipitation required
- No cumbersome steps associated with resins and slurries
- 40% more preps per kit than the leading supplier at a similar cost
- Fast and Simple - DNA fragments purified in 15 minutes
- Up to 80% recovery of DNA fragments (50 bp-10 kb)
- DNA is ready suitable for all common downstream applications
- No toxic organic solvent extraction or precipitation
- No cumbersome steps associated with resins and slurries
Preparation Note
Sufficient for 70 preparations.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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