P2783
Phosphotransacetylase from Bacillus stearothermophilus
lyophilized powder, ≥3,000 units/mg protein (biuret)
别名:
Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-855-2
MDL number:
Specific activity:
≥3,000 units/mg protein (biuret)
Application
Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.
Biochem/physiol Actions
Phosphotransacetylase converts CoA to acetyl CoA.
Other Notes
One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.
Physical form
Lyophilized powder containing potassium phosphate
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
Philip R. Mallinder and Anne Moir
Microbiology, 137, 779-785 (1991)
G D Sprott et al.
Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
Maria Dimou et al.
Molecular biology reports, 38(6), 3653-3663 (2010-11-26)
Acetate is abundant in soil contributing to a great extent on carbon cycling in nature. Phosphate acetyltransferase (Pta, EC 2.3.1.8) catalyzes the reversible transfer of the acetyl group from acetyl-P to CoA forming acetyl-CoA and inorganic phosphate, participating to acetate
Ikuo Sato et al.
Bioscience, biotechnology, and biochemistry, 74(1), 75-81 (2010-01-09)
Malic enzyme (ME) was purified as an electrophoretically homogenous protein from Rhodopseudomonas palustris No. 7. The molecular weight of ME was estimated to be 650 kDa and that of its subunit, 86 kDa. ME activity was remarkably enhanced by di-
Guiqing Yang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 26(2), 170-176 (2010-05-04)
Clostridium tyrobutyricum is suitable for simultaneous saccharification and fermentation of lignocellulosic. It can produce butyric acid, acetic acid as its main fermentation products from a wide variety of carbohydrates such as glucose, xylose, cellobiose and arabinose. In order to decrease
商品
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
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