P2783
Phosphotransacetylase from Bacillus stearothermophilus
lyophilized powder, ≥3,000 units/mg protein (biuret)
别名:
Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase
表单
lyophilized powder
比活
≥3,000 units/mg protein (biuret)
组成
Protein, ≥20%
运输
wet ice
储存温度
−20°C
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应用
Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.
生化/生理作用
Phosphotransacetylase converts CoA to acetyl CoA.
外形
Lyophilized powder containing potassium phosphate
其他说明
One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
G D Sprott et al.
Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
Maria Dimou et al.
Molecular biology reports, 39(4), 4135-4143 (2011-07-21)
Cyclophilins belong to the peptidyl-prolyl cis/trans isomerase family of enzymes (EC 5.2.1.8), which accelerate protein folding by catalysing the cis/trans isomerisation of proline imidic peptide bonds. In the present study, by a combination of bioinformatics methods, we identify phosphate acetyltransferase
Zoltán Gregus et al.
Toxicological sciences : an official journal of the Society of Toxicology, 110(2), 282-292 (2009-05-30)
Enzymes catalyzing the phosphorolytic cleavage of their substrates can reduce arsenate (AsV) to the more toxic arsenite (AsIII) via the arsenolytic substrate cleavage in presence of a reductant, as glutathione or dithiotreitol (DTT). We have shown this for purine nucleoside
Shital A Tripathi et al.
Applied and environmental microbiology, 76(19), 6591-6599 (2010-08-10)
We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene.
Jing Huang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 480-487 (2011-07-30)
To study the effects of gene pta disruption on biosynthesis of L-tryptophan. The pta gene of the L-tryptophan producing strain E. coli TRTH was disrupted by Red recombination technology and a pta mutant E. coli TRTHdeltapta was constructed. Fed-batch fermentation
商品
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
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