P3397
磷酸葡萄糖变位酶 来源于兔肌肉
ammonium sulfate suspension, ≥100 units/mg protein
别名:
PGM, α-D-葡萄糖-1,6-二磷酸酶, α-D-葡萄糖磷酸转移酶
生物来源
rabbit muscle
质量水平
描述
ammonium sulfate suspension, >=100 units/mg protein
表单
ammonium sulfate suspension
比活
≥100 units/mg protein
储存条件
(Tightly closed)
技术
activity assay: suitable
颜色
white to light yellow
异质活性
lactic dehydrogenase ≤0.5%
phosphoglucose isomerase ≤0.01%
pyruvate kinase ≤0.05%
运输
wet ice
储存温度
2-8°C
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一般描述
适合研究领域：细胞信号传导
磷酸葡萄糖变位酶(PGM)，是一种保守酶，在动物、植物和微生物中大量存在，几乎分布于所有组织中。
磷酸葡萄糖变位酶(PGM)，是一种保守酶，在动物、植物和微生物中大量存在，几乎分布于所有组织中。
应用
本品（磷酸葡萄糖变位酶）已用于：
- 研究人类的糖原合成与磷酸葡萄糖变位酶缺乏
- 通过检测磷酸葡萄糖变位酶的活性研究代谢调控
- 试验A（参见文献），检测BL21(DE3)C43转化细胞中的糖原代谢密切相关酶
- 蔗糖合酶(SuSy)和ADP葡萄糖焦磷酸化酶(AGPase)检测(3)
- 在酶活性测定中结合烟酰胺腺嘌呤二核苷酸磷酸(NADP+)还原反应，测定来自蔗糖和无机磷酸(Pi)的葡萄糖-1-磷酸
生化/生理作用
磷酸葡萄糖变位酶(PGM)在糖酵解和糖异生中起着至关重要的作用。参与糖原和昆虫海藻糖的代谢。磷酸葡萄糖变位酶也参与植物和某些微生物的发育。葡萄糖-6-磷酸是核心代谢产物，因此PGM是蛋白质、脂质和核酸的代谢关键，对植物发育至关重要。磷酸葡萄糖变位酶催化葡萄糖-6-磷酸(G-6-P)和葡萄糖-1-磷酸(G-1-P)的相互转变。
外形
(NH4)2SO4，pH 6.0，含 0.01%EDTA 的晶体混悬液
分析说明
缩二脲法测定蛋白
其他说明
1 个单位将在 30°C、pH 7.4 下每分钟将 1.0 μmolα-D-葡萄糖 1-磷酸转化为 α-D-葡萄糖 6-磷酸。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
H Sugie et al.
Neurology, 38(4), 602-605 (1988-04-01)
We report a 5-month-old boy with recurrent vomiting, lethargy, and poor weight gain. He had profound metabolic acidosis and nonketotic dicarboxylic aciduria. The serum and muscle carnitine levels were significantly low (60% and 10% of the control means, respectively), suggesting
Nora Alonso-Casajús et al.
Journal of bacteriology, 188(14), 5266-5272 (2006-07-04)
To understand the biological function of bacterial glycogen phosphorylase (GlgP), we have produced and characterized Escherichia coli cells with null or altered glgP expression. glgP deletion mutants (DeltaglgP) totally lacked glycogen phosphorylase activity, indicating that all the enzymatic activity is
Bi-Ying Pan et al.
3 Biotech, 10(2), 61-61 (2020-02-08)
Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1
Leszek A Kleczkowski et al.
Plants (Basel, Switzerland), 11(12) (2022-06-24)
UDP-glucose pyrophosphorylase (UGPase) carries a freely reversible reaction, using glucose-1-P and UTP to produce UDP-glucose (UDPG) and pyrophosphate (PPi), with UDPG being essential for glycosylation reactions in all organisms including, e.g., synthesis of sucrose, cellulose and glycoproteins. In the present
Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli
Lee Ha J, et al.
Enzyme and Microbial Technology, 39, 612-620 (2006)
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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