P4377
碱性磷酸酶 来源于大肠杆菌
ammonium sulfate suspension, ~10 units/mg protein (modified Warburg-Christian)
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-631-4
MDL number:
eCl@ss:
42010105
form
ammonium sulfate suspension
specific activity
~10 units/mg protein (modified Warburg-Christian)
storage temp.
2-8°C
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4377 has been used for cell digestion during measurement of photoreactivating enzyme
Biochem/physiol Actions
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Physical form
Suspension in 2.5 M (NH4)2SO4 solution
Preparation Note
Salt fractionated
Other Notes
一个单位将在37°C 、 pH 10.4 下每分钟水解 1.0μ摩尔 对 -硝基苯磷酸盐。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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相关内容
QC Methods
B M Sutherland et al.
Journal of bacteriology, 138(2), 333-338 (1979-05-01)
Sutherland et al. mapped a phr gene in Escherichia coli at 17 min and found that induction of an E. coli strain lysogenic for a lambda phage carrying this gene increased photoreactivating enzyme levels 2,000-fold. Recently, Smith and Youngs and
Naomi Ofer et al.
The Journal of biological chemistry, 288(16), 11287-11293 (2013-03-09)
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell