一般描述
天然完整的人纤溶酶原是791个氨基酸的糖蛋白，具有多达 24个二硫键。纤溶酶原包含单个N-连接的唾液酸化双触角聚糖。两个O-聚糖具有Gal β-1-3GalNAc 核心，其在末端Gal 处是α-2-3唾液酸化的。另外的二唾液酸化形式具有第二唾液酸残基，其与GalNAc 具有α-2-6键。单 -和二唾液酸化形式在人纤溶酶原中以 80：20的摩尔比发生。
生化/生理作用
纤溶酶原是蛋白酶纤溶酶的无活性前体。纤溶酶原由组织型纤溶酶原激活剂 (tPA) 激活，tPA 主要激活纤溶酶的纤维蛋白溶解（血栓溶解）活性，或者由尿激酶型纤溶酶原激活剂 (uPA) 激活，uPA 与细胞外基质重塑和细胞迁移有关。纤溶酶可裂解纤维蛋白/纤维蛋白原和凝血因子 V/Va 以及 VIII/VIIIa。它可通过裂解非活性酶原激活基质金属蛋白酶。还与某些生长因子的激活有关，如血管內皮生长因子 (VEGF) 和转化生长因子 β (TGF-β)。
外形
冻干粉，含 NaCl、EDTA、赖氨酸以及 Tris 缓冲盐
制备说明
溶液应储存在含 20mM 赖氨酸的中性 pH 缓冲液中。蛋白质应尽量浓缩。分成小份在 -20°C 下冻存，可稳定保存几周。
分析说明
不含 ε-氨基己酸
经检测，每位捐献者的血浆 HIV-1/HIV-2 抗体、HCV 和 HbSAg 抗体均为阴性。
其他说明
当测定 5.0mL 体积中的高氯酸可溶性产物时，在 pH 7.5 和 37°C 下，一单位本品将使 α-酪蛋白在 20 分钟内产生 1.0 的 ΔA275。活性在尿激酶激活纤溶酶后测定。
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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