biological source
human plasma
form
lyophilized powder
specific activity
≥2 units/mg protein
mol wt
90 kDa
composition
, ~2%
concentration
~2% protein (Lowry)
suitability
not suitable for streptokinase determination by clot formation procedure
UniProt accession no.
foreign activity
plasmin <20% of base activity
storage temp.
−20°C
Gene Information
human ... PLG(5340)
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General description
天然完整的人纤溶酶原是791个氨基酸的糖蛋白，具有多达 24个二硫键。纤溶酶原包含单个N-连接的唾液酸化双触角聚糖。两个O-聚糖具有Gal β-1-3GalNAc 核心，其在末端Gal 处是α-2-3唾液酸化的。另外的二唾液酸化形式具有第二唾液酸残基，其与GalNAc 具有α-2-6键。单 -和二唾液酸化形式在人纤溶酶原中以 80：20的摩尔比发生。
Biochem/physiol Actions
纤溶酶原是蛋白酶纤溶酶的无活性前体。纤溶酶原由组织型纤溶酶原激活剂 (tPA) 激活，tPA 主要激活纤溶酶的纤维蛋白溶解（血栓溶解）活性，或者由尿激酶型纤溶酶原激活剂 (uPA) 激活，uPA 与细胞外基质重塑和细胞迁移有关。纤溶酶可裂解纤维蛋白/纤维蛋白原和凝血因子 V/Va 以及 VIII/VIIIa。它可通过裂解非活性酶原激活基质金属蛋白酶。还与某些生长因子的激活有关，如血管內皮生长因子 (VEGF) 和转化生长因子 β (TGF-β)。
Physical form
冻干粉，含 NaCl、EDTA、赖氨酸以及 Tris 缓冲盐
Preparation Note
溶液应储存在含 20mM 赖氨酸的中性 pH 缓冲液中。蛋白质应尽量浓缩。分成小份在 -20°C 下冻存，可稳定保存几周。
Analysis Note
不含 ε-氨基己酸
经检测，每位捐献者的血浆 HIV-1/HIV-2 抗体、HCV 和 HbSAg 抗体均为阴性。
Other Notes
当测定 5.0mL 体积中的高氯酸可溶性产物时，在 pH 7.5 和 37°C 下，一单位本品将使 α-酪蛋白在 20 分钟内产生 1.0 的 ΔA275。活性在尿激酶激活纤溶酶后测定。
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Barbara Ruszkowska et al.
Acta obstetricia et gynecologica Scandinavica, 92(7), 841-846 (2013-03-12)
To assess the concentration of adiponectin, soluble E-selectin, soluble thrombomodulin and tissue activator plasminogen antigen in postmenopausal women who received oral or transdermal hormone therapy. Case-control study. Polish university hospitals. Seventy-six healthy postmenopausal women. Forty-six women who received oral (n
Cen Li et al.
Cornea, 32(5), 680-688 (2013-01-25)
Recent results showed that plasminogen kringle 5 (K5) has improved inhibitory effect on human umbilical vein endothelial cells (HUVECs) viability when 5 acidic amino acids in NH2 terminal outside kringle domain were replaced by 5 serine residues (mutant K5, mK5).
Yueling Zhang et al.
FEBS letters, 587(9), 1304-1309 (2013-03-12)
Cluster 1 streptokinases (SK1) from Streptococcus pyogenes (GAS) show substantially higher human plasminogen (hPg) activation activities and tighter hPg binding affinities than cluster 2b streptokinases (SK2b) in solution. The extent to which the different domains of SK are responsible for
Venous thrombosis in the nephrotic syndrome.
Joseph Loscalzo
The New England journal of medicine, 368(10), 956-958 (2013-03-08)
Liver-artery interactions via the plasminogen-CD36 axis in macrophage foam cell formation: new evidence for the role of remote organ crosstalk in atherosclerosis.
Hiroshi Iwata et al.
Circulation, 127(11), 1173-1176 (2013-03-20)
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