form
liquid
technique(s)
flow cytometry: suitable
fluorescence
λex 490 nm; λem 504 nm (PKH2)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Application
The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for more than 21 days in vivo. Intraperitoneal or intravenous injections of the PKH2 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.
Other Notes
For additional technical details on PKH Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit www.sigma-aldrich.com/pkh.
Legal Information
Distributed for Phanos Technologies.
仅试剂盒组分
产品编号
说明
- Diluent B 6 x 10
- PKH2 Cell Linker in ethanol .5 mL
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
M J Melnicoff et al.
Cellular immunology, 118(1), 178-191 (1989-01-01)
The kinetics of macrophage (M phi) recruitment to the peritoneum following the induction of acute inflammation by thioglycollate broth (TG) was evaluated after prelabeling resident M phi with the fluorescent cell tracking dye, PKH-1. Most of the PKH-1-labeled resident M
Fluorescent cell labeling for in vivo and in vitro cell tracking.
P K Horan et al.
Methods in cell biology, 33, 469-490 (1990-01-01)
M J Melnicoff et al.
Journal of leukocyte biology, 43(5), 387-397 (1988-05-01)
A novel method for labeling resident peritoneal macrophages (M phi) by injection of a dye into the peritoneal cavity is described. The dye, which fluoresces green, is selectively taken up by the resident M phi. Dye labeled cells can be
Rachel T van Beem et al.
Journal of immunology (Baltimore, Md. : 1950), 180(7), 5141-5148 (2008-03-21)
The number of colony forming unit-endothelial cells (CFU-EC) in human peripheral blood was found to be a biological marker for several vascular diseases. In this study, the heterogeneous composition of immune cells in the CFU-ECs was investigated. We confirmed that
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