R0525
RANK 配体 来源于小鼠
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
NF-κB 配体受体激活剂（RANKL）, 破骨细胞分化因子（ODF）, 肿瘤坏死因子相关的激活−诱导细胞因子（TRANCE）, 骨保护素配体（OPGL）
产品名称
RANK 配体 来源于小鼠, ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
10.0-25.0 ng/mL ED50
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/mg
<1 EU/μg
color
white
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
mouse ... Tnfsf11(21943)
Analysis Note
生物活性由其诱导 RAW 264.7的破骨细胞分化的能力决定。该效果的预期 ED50 为≤10ng/ml。
Application
RANK配体的功能包括诱 c-jun N末端激酶的活化、增强T细胞生长和破骨细胞生成的树突细胞功能诱导，以及淋巴结器官发生。RANK 配体的细胞表面信号传导受体是RANK，其在信号转导过程中的经受受体聚集。
小鼠 RANK 配体已用于可视化分化的破骨细胞，并刺激破骨细胞分化。
Biochem/physiol Actions
肿瘤坏死因子（配体）超家族成员 11（Tnfsf11）或 RANKL在破骨细胞功能和分化中具有重要作用。它与RANK和骨保护素（OPG）结合。RANKL的生物活性可归因于它与这两种受体分子的结合。小鼠和人RANKL有85%的氨基酸序列同源性。
General description
肿瘤坏死因子（配体）超家族成员 11（Tnfsf11）或核因子-κB 配体受体激活剂（RANKL）是II型跨膜信号传导受体。它的分子量约为35kDa。该蛋白质具有氨基末端的细胞内尾部和羧基末端的细胞外区域，其具有连接茎和受体结合结构域。
Physical form
从 0.2 μm 过滤的5mM 磷酸钠，pH 7.6和75mM NaCl的溶液中冻干。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
RANKL employs distinct binding modes to engage RANK and the OPG decoy receptor
Christopher A
Structure (2012)
Ningning Jiang et al.
Biological trace element research, 193(1), 226-233 (2019-03-17)
Fluoride accumulates and is toxic to bones. Clinical bone lesions occur in a phased manner, being less severe early in the natural course of skeletal fluorosis. Previous research rarely focused on osteocyte, osteoclast, and osteoblast at the same time, although
Erin L Ealba et al.
Developmental biology, 408(1), 151-163 (2015-10-10)
Precise control of jaw length during development is crucial for proper form and function. Previously we have shown that in birds, neural crest mesenchyme (NCM) confers species-specific size and shape to the beak by regulating molecular and histological programs for
Matrine prevents bone loss in ovariectomized mice by inhibiting RANKL-induced osteoclastogenesis
Xiao Chen
Faseb Journal (2017)
Elizabeth Rendina-Ruedy et al.
Journal of bone and mineral metabolism, 34(4), 380-394 (2015-06-11)
Skeletal fractures are considered a chronic complication of type 2 diabetes mellitus (T2DM), but the etiology of compromised bone quality that develops over time remains uncertain. This study investigated the concurrent alterations in metabolic and skeletal changes in two mouse
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