等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
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应用
SalI is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.
生化/生理作用
Recognition sequence: 5′-G/TCGAC-3′
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
外形
Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 50% glycerol (v/v), 10 mM dithioerythritol, at 4 °C
其他说明
One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 ml of buffer SH for restriction enzymes.
Supplied with 10x Restriction Enzyme Buffer SH (B3657)
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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In situ preservation of DNA in plant species.
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Taxon, 38, 576-576 (1989)
Y Katayama et al.
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We have previously shown that the methicillin-resistance gene mecA of Staphylococcus aureus strain N315 is localized within a large (52-kb) DNA cassette (designated the staphylococcal cassette chromosome mec [SCCmec]) inserted in the chromosome. By sequence determination of the entire DNA
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Journal of molecular biology, 118(1), 127-135 (1978-01-05)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
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