表单
buffered aqueous glycerol solution
浓度
5,000 units/mL
运输
wet ice
储存温度
−20°C
生化/生理作用
Recognition Sequence: 5′-A/CPuPyGT-3′
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
外形
Solution in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1mM EDTA, 200 ug/ml BSA, 0.15% Triton X-100, 50% Glycerol (v/v) , pH 8.0 at 4°C
其他说明
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
法规信息
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P R Whitehead et al.
Journal of general microbiology, 131(4), 951-958 (1985-04-01)
Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: (formula; see text) AflII and AflIII are new specificities and may be
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
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