form
buffered aqueous glycerol solution
concentration
5,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition Sequence: 5′-A/CPuPyGT-3′
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Physical form
Solution in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1mM EDTA, 200 ug/ml BSA, 0.15% Triton X-100, 50% Glycerol (v/v) , pH 8.0 at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
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P R Whitehead et al.
Journal of general microbiology, 131(4), 951-958 (1985-04-01)
Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: (formula; see text) AflII and AflIII are new specificities and may be
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Lilia Gabsalilow et al.
Nucleic acids research, 41(7), e83-e83 (2013-02-15)
Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions
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