等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
应用
EcoRV is a DNA restriction endonuclease used in molecular biology applications to cleave the recognition site 5′-GAT/ATC-3′, generating DNA fragments with blunt termini.
生化/生理作用
Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
外形
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 0.1 mM PMSF, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 0.1 mM PMSF, 50% glycerol (v/v) at 4°C
其他说明
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Stability of transformed antagonistic Fusarium oxysporum strains in vitro and in soil microcosms.
Migheli, Q., et al.
Molecular Ecology, 5, 641-641 (1996)
I Schildkraut et al.
Gene, 27(3), 327-329 (1984-03-01)
The cleavage site for the restriction endonuclease EcoRV has been found to be 5'-GAT/ATC-3', rather than 5'- GATAT /C-3' as reported earlier by Kholmina et al. [ Dokl . Akad . Nauk . 253 (1980) 495-497].
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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