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Merck
CN

R5257

Spe I from Sphaerotilus sp.

Restriction Enzyme

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化学文摘社编号:
UNSPSC Code:
12352204
MDL number:
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grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Application

SpeI is a restriction endonuclease used for molecular biology applications to cut DNA at the recognition site 5′-A/CTAGT-3′, generating DNA fragments with 5′-cohesive ends.

Biochem/physiol Actions

Recognition sequence: 5′-A/CTAGT-3′
Cutting results: a 2-10-fold Spe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0 , 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

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Cracraft, J., et al.
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Restriction and modification enzymes and their recognition sequences.
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Journal of clinical microbiology, 40(11), 4051-4055 (2002-11-01)
Successful carbapenem-based chemotherapy for the treatment of Pseudomonas infections has been seriously hindered by the recent appearance of IMP- and VIM-type metallo-beta-lactamases, which confer high-level resistance to carbapenems and most other beta-lactams. Recently, multidrug-resistant Pseudomonas putida isolates for which carbapenem
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis

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