等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
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应用
Eco RI 是一种限制性内切酶，在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′，产生带有 5′ 的片段-粘性终点。
生化/生理作用
识别序列： 5′-G/AATTC-3′
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
外形
10 mM Tris-HCl 溶液，pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v)，4°C
其他说明
备注： 避免次优反应条件，如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%)，因为它们将改变 EcoRI 特异性并沉淀星号活性。
一个单位表示在37°C下1小时内，在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
提供 10x 限制性内切酶缓冲液 SH (B3657)。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
J Hedgpeth et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(11), 3448-3452 (1972-11-01)
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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Yu-Feng Huang et al.
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Nico Mitro et al.
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