R8000
D-核糖1,5-二磷酸羧化酶 来源于菠菜
partially purified powder, 0.01-0.1 unit/mg solid
别名:
3-磷酸-D-甘油酸羧裂合酶(二聚), Rubisco
相关类别
一般描述
D-核酮糖1,5-二磷酸羧化酶(RUBISCO)占菠菜叶可溶蛋白总量的50%。
以一种557 kDa hexadecamer存在，它由8个分子量约为56 kDa的重链以及8个分子量约为14 kDa的轻链组成。每个分子包含一个镁离子。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
应用
菠菜D-核酮糖1,5-二磷酸羧化酶已用于：
- 作为胃蛋白酶消化研究的测试蛋白
- 检测其作为无害蛋白样品，对人肠上皮细胞系的影响
- 和脱落酸的等温滴定量热法(ITC)和放射性标记结合检测
生化/生理作用
D-核酮糖1,5-二磷酸羧化酶（RUBISCO，核酮糖二磷酸羧化酶）的激活依赖Rubisco激活酶和分子伴侣。它参与植物光吸收事件，可催化核酮糖1,5-二磷酸的羧化和加氧。Rubisco酶的羧化活性受脱落酸抑制。
其他说明
在pH 7.8、25°C条件下，一单位可在每分钟将1.0 μmole的D-RuDP和CO2转化为2.0 μmole的D-3-磷酸甘油酸酯。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Shunsuke Adachi et al.
Journal of experimental botany, 64(4), 1061-1072 (2013-01-26)
Increases in rates of individual leaf photosynthesis (P(n)) are critical for future increases in yields of rice plants. Although many efforts have been made to improve rice P(n) with transgenic technology, the desired increases in P(n) have not yet been
Benjamin D Rae et al.
Microbiology and molecular biology reviews : MMBR, 77(3), 357-379 (2013-09-06)
Cyanobacteria are the globally dominant photoautotrophic lineage. Their success is dependent on a set of adaptations collectively termed the CO2-concentrating mechanism (CCM). The purpose of the CCM is to support effective CO2 fixation by enhancing the chemical conditions in the
Guillaume Tcherkez
Plant, cell & environment, 36(9), 1586-1596 (2013-01-12)
Although ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was discovered nearly 60 years ago, the associated chemical mechanism of the reaction is still incompletely understood. The catalytic cycle consists of four major steps: ribulose-1,5-bisphosphate binding, enolization, CO₂ or O₂ addition and hydration, and cleavage
Martin A J Parry et al.
Journal of experimental botany, 64(3), 717-730 (2012-11-20)
Rubisco (ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase) enables net carbon fixation through the carboxylation of RuBP. However, some characteristics of Rubisco make it surprisingly inefficient and compromise photosynthetic productivity. For example, Rubisco catalyses a wasteful reaction with oxygen that leads to the release
K Thomas et al.
Regulatory toxicology and pharmacology : RTP, 39(2), 87-98 (2004-03-26)
Rationale. Evaluation of the potential allergenicity of proteins derived from genetically modified foods has involved a weight of evidence approach that incorporates an evaluation of protein digestibility in pepsin. Currently, there is no standardized protocol to assess the digestibility of
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