R8000
D-核糖1,5-二磷酸羧化酶 来源于菠菜
partially purified powder, 0.01-0.1 unit/mg solid
别名:
3-磷酸-D-甘油酸羧裂合酶(二聚), Rubisco
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
0.01-0.1 unit/mg solid
Biological source:
spinach
biological source
spinach
Quality Level
form
partially purified powder
specific activity
0.01-0.1 unit/mg solid
mol wt
557 kDa
storage temp.
−20°C
General description
D-核酮糖1,5-二磷酸羧化酶(RUBISCO)占菠菜叶可溶蛋白总量的50%。
以一种557 kDa hexadecamer存在，它由8个分子量约为56 kDa的重链以及8个分子量约为14 kDa的轻链组成。每个分子包含一个镁离子。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
Application
菠菜D-核酮糖1,5-二磷酸羧化酶已用于：
- 作为胃蛋白酶消化研究的测试蛋白
- 检测其作为无害蛋白样品，对人肠上皮细胞系的影响
- 和脱落酸的等温滴定量热法(ITC)和放射性标记结合检测
Biochem/physiol Actions
D-核酮糖1,5-二磷酸羧化酶（RUBISCO，核酮糖二磷酸羧化酶）的激活依赖Rubisco激活酶和分子伴侣。它参与植物光吸收事件，可催化核酮糖1,5-二磷酸的羧化和加氧。Rubisco酶的羧化活性受脱落酸抑制。
Other Notes
在pH 7.8、25°C条件下，一单位可在每分钟将1.0 μmole的D-RuDP和CO2转化为2.0 μmole的D-3-磷酸甘油酸酯。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
K Thomas et al.
Regulatory toxicology and pharmacology : RTP, 39(2), 87-98 (2004-03-26)
Rationale. Evaluation of the potential allergenicity of proteins derived from genetically modified foods has involved a weight of evidence approach that incorporates an evaluation of protein digestibility in pepsin. Currently, there is no standardized protocol to assess the digestibility of
Martin A J Parry et al.
Journal of experimental botany, 64(3), 717-730 (2012-11-20)
Rubisco (ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase) enables net carbon fixation through the carboxylation of RuBP. However, some characteristics of Rubisco make it surprisingly inefficient and compromise photosynthetic productivity. For example, Rubisco catalyses a wasteful reaction with oxygen that leads to the release
Dennis McNevin et al.
Journal of experimental botany, 57(14), 3883-3900 (2006-10-19)
The forward and reverse rate constants involved in carbamylation, activation, carboxylation, and inhibition of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) have been estimated by a new technique of simultaneous non-linear regression of a differential equation kinetic model to multiple experimental data. Parameters predicted
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R8000-5UN | 04061836807375 |
| R8000-1UN | 04061836807368 |