grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
NotI is used in molecular biology methods as a DNA restriction enzyme that cuts DNA at the recognition site 5′-GC/GGCCGC-3′ to generate DNA fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-GC/GGCCGC-3′
Cutting results: a 2-10-fold Not I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Not I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4 °C.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Restriction and modification enzymes and their recognition sequences.
R J Roberts
Nucleic acids research, 12 Suppl, r167-r204 (1984-01-01)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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