R9507
Taq I 来源于水生栖热菌
recombinant, expressed in E. coli (Strain that carries a Taq I overproducing plasmid.), Restriction Enzyme
重组
expressed in E. coli (Strain that carries a Taq I overproducing plasmid.)
等级
Molecular Biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
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应用
TaqI is a restriction endonuclease used in molecular biology applications to cleave DNA moledcules at the recognition site 5′-T/CGA-3′, generating fragments with 5′-cohesive ends.
生化/生理作用
Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
外形
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4 °C
其他说明
Comment: Taq I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). Taq I is inefficient in digesting single stranded DNA . Overlapping dam methylation will block cleavage by Taq I. Activity of the enzyme is enhanced by BSA and higher temperatures, >37 °C. Optimal temperature is 65 °C.
Supplied with 10x Restriction Enzyme Buffer SB (B8781).
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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S Sato et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 542-546 (1977-02-01)
A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and bacteriophage psiX174 RF DNA at 10 sites. The enzyme
Y Gruenbaum et al.
Nucleic acids research, 9(11), 2509-2515 (1981-06-11)
Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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