S0937
Sucrose Phosphorylase
recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid
别名:
SPase, 二糖葡糖基转移酶, 蔗糖转葡糖基酶,
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥45 units/mg solid
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥45 units/mg solid
mol wt
56 kDa by SDS-PAGE
shipped in
wet ice
storage temp.
−20°C
General description
研究领域：细胞信号传导
蔗糖磷酸化酶属于糖苷水解酶GH13家族。它由四个结构域组成，包括葡萄糖异头碳结合位点和糖苷结合位点。活性位点残基包括Asp192和Glu232。它主要由双歧杆菌和乳酸杆菌产生。交联的蔗糖磷酸化酶聚集体具有热稳定性，可用于工业糖基化催化。
蔗糖磷酸化酶属于糖苷水解酶GH13家族。它由四个结构域组成，包括葡萄糖异头碳结合位点和糖苷结合位点。活性位点残基包括Asp192和Glu232。它主要由双歧杆菌和乳酸杆菌产生。交联的蔗糖磷酸化酶聚集体具有热稳定性，可用于工业糖基化催化。
Application
蔗糖磷酸化酶已用于测定小麦植株中的蔗糖 和蔗糖氢的生产。
蔗糖磷酸化酶已用于：
- 评估稳定、无味且呈粉末状的呋喃酮糖苷的酶促合成。
- 研究与羧酸化合物的新型转糖基化反应。
- 用于小麦植株中的蔗糖测定和蔗糖制氢。
Biochem/physiol Actions
蔗糖磷酸化酶催化蔗糖（α-D-吡喃葡萄糖基-1,2-β-D-呋喃果糖苷）的可逆转化和将磷酸盐催化成D-果糖和α-D-葡萄糖1-磷酸。此反应在通过蔗糖代谢产生至关重要的葡萄糖组分方面起着关键作用。(1)
Physical form
含有作为稳定剂的蔗糖。
Other Notes
在 25°C和pH 7.6下，一个单位每分钟将从蔗糖产生 1.0μ摩尔 D 果糖，同时相应地将NADP还原为NADPH。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
常规特殊物品
此项目有
Jin-Ha Lee et al.
Biotechnology letters, 30(4), 749-754 (2007-11-27)
The gene encoding sucrose phosphorylase (742sp) in Leuconostoc mesenteroides NRRL B-742 was cloned and expressed in Escherichia coli. The nucleotide sequence of the transformed 742sp comprised an ORF of 1,458 bp giving a protein with calculated molecular mass of 55.3
Kuniki Kino et al.
Bioscience, biotechnology, and biochemistry, 72(9), 2415-2417 (2008-09-09)
Cellobiose phosphorylase from Clostridium thermocellum catalyzed the beta-anomer-selective synthesis of alkyl glucosides from cellobiose. Synthesis of alkyl beta-glucoside from inexpensive sucrose using cellobiose phosphorylase and sucrose phosphorylase from Pseudomonas saccharophilia was investigated. By combined use of these two phosphorylases, alkyl
Genotypic variation in water-soluble carbohydrate accumulation in wheat
Ruuska SA, et al.
Functional plant biology, 33(9), 799-809 (2006)
Alexandra Schwarz et al.
The Biochemical journal, 403(3), 441-449 (2007-01-20)
The role of acid-base catalysis in the two-step enzymatic mechanism of alpha-retaining glucosyl transfer by Leuconostoc mesenteroides sucrose phosphorylase has been examined through site-directed replacement of the putative catalytic Glu237 and detailed comparison of purified wild-type and Glu237-->Gln mutant enzymes
Koji Nomura et al.
Bioscience, biotechnology, and biochemistry, 72(1), 82-87 (2008-01-08)
Transglucosylation from sucrose to acetic acid by sucrose phosphorylase (EC 2.4.1.7) was studied. 1-O-Acetyl-alpha-D-glucopyranose was isolated as the main product of the enzyme reaction. We also compared the pH-dependence of transglycosylation catalyzed by sucrose phosphorylase toward carboxyl and hydroxyl groups.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S0937-250UN | 04061832925394 |
| S0937-1000UN | 04061832925387 |
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