S0937
Sucrose Phosphorylase
recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid
别名:
SPase, 二糖葡糖基转移酶, 蔗糖转葡糖基酶,
产品名称
Sucrose Phosphorylase, recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid
recombinant
expressed in E. coli
form
lyophilized powder
specific activity
≥45 units/mg solid
mol wt
56 kDa by SDS-PAGE
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
蔗糖磷酸化酶已用于测定小麦植株中的蔗糖 和蔗糖氢的生产。
蔗糖磷酸化酶已用于：
- 评估稳定、无味且呈粉末状的呋喃酮糖苷的酶促合成。
- 研究与羧酸化合物的新型转糖基化反应。
- 用于小麦植株中的蔗糖测定和蔗糖制氢。
Biochem/physiol Actions
蔗糖磷酸化酶催化蔗糖（α-D-吡喃葡萄糖基-1,2-β-D-呋喃果糖苷）的可逆转化和将磷酸盐催化成D-果糖和α-D-葡萄糖1-磷酸。此反应在通过蔗糖代谢产生至关重要的葡萄糖组分方面起着关键作用。(1)
General description
研究领域：细胞信号传导
蔗糖磷酸化酶属于糖苷水解酶GH13家族。它由四个结构域组成，包括葡萄糖异头碳结合位点和糖苷结合位点。活性位点残基包括Asp192和Glu232。它主要由双歧杆菌和乳酸杆菌产生。交联的蔗糖磷酸化酶聚集体具有热稳定性，可用于工业糖基化催化。
蔗糖磷酸化酶属于糖苷水解酶GH13家族。它由四个结构域组成，包括葡萄糖异头碳结合位点和糖苷结合位点。活性位点残基包括Asp192和Glu232。它主要由双歧杆菌和乳酸杆菌产生。交联的蔗糖磷酸化酶聚集体具有热稳定性，可用于工业糖基化催化。
Other Notes
在 25°C和pH 7.6下，一个单位每分钟将从蔗糖产生 1.0μ摩尔 D 果糖，同时相应地将NADP还原为NADPH。
Physical form
含有作为稳定剂的蔗糖。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
常规特殊物品
此项目有
Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate.
Christiane Goedl et al.
Chembiochem : a European journal of chemical biology, 10(14), 2333-2337 (2009-08-20)
Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion
Mirza O, et al.
The Journal of Biological Chemistry (2006)
Christiane Goedl et al.
Carbohydrate research, 343(12), 2032-2040 (2008-03-19)
Sucrose phosphorylase utilizes a glycoside hydrolase-like double displacement mechanism to convert its disaccharide substrate and phosphate into alpha-d-glucose 1-phosphate and fructose. Site-directed mutagenesis was employed to characterize the proposed roles of Asp(196) and Glu(237) as catalytic nucleophile and acid-base, respectively
A Kasperowicz et al.
Journal of applied microbiology, 107(3), 812-820 (2009-03-27)
To verify the taxonomic affiliation of bacterium Butyrivibrio fibrisolvens strain A from our collection and to characterize its enzyme(s) responsible for digestion of sucrose. Comparison of the 16S rRNA gene of the bacterium with GenBank showed over 99% sequence identity
Thornthan Sawangwan et al.
Organic & biomolecular chemistry, 7(20), 4267-4270 (2009-10-02)
Regioselective glucosylation of R-glycerate catalysed by sucrose phosphorylase in the presence of sucrose as the donor substrate provided the natural compatible solute (R)-2-O-alpha-D-glucopyranosyl glycerate with complete regioselectivity in an optimised synthetic yield of 90% R-glycerate converted and a concentration of
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