form
solution
specific activity
10-40 (units/ml)
shipped in
dry ice
storage temp.
−20°C
General description
Hydrolyses the terminal 2-O-sulphate on the unsaturated di- and oligo-saccharides produced by the action of lyases on sulphated glycosaminoglycans.
Application
2-O-Sulfatase from Flavobacterium heparinum has been used in a study to determine that oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events. 2-O-Sulfatase from Flavobacterium heparinum has also been used in a study to determine the structure of oligosaccharides prepared from acharan sulfate.
Physical form
Solution contains 10 mM tris-acetate, pH 7.3, 80 mM NaCl, 10 mM potassium phosphate, 0.2% BSA.
Other Notes
One unit will hydrolyze heparin disaccharide I-P (H9401) to yield 1.0 micromole of disaccharide II-P (δ-UA-GlcNCOEt-6S) per minute at pH 7.3 at 25 °C.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
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Rahul Raman et al.
The Journal of biological chemistry, 278(14), 12167-12174 (2003-01-10)
In the previous paper (Myette, J. R., Shriver, Z., Claycamp, C., McLean, M. W., Venkataraman, G., and Sasisekharan, R. (2003) J. Biol. Chem. 278, 12157-12166), we described the molecular cloning, recombinant expression, and preliminary biochemical characterization of the heparin/heparan sulfate
Marco Guerrini et al.
Nature biotechnology, 26(6), 669-675 (2008-04-26)
Recently, certain lots of heparin have been associated with an acute, rapid onset of serious side effects indicative of an allergic-type reaction. To identify potential causes for this sudden rise in side effects, we examined lots of heparin that correlated
Y S Kim et al.
Glycobiology, 8(9), 869-877 (1998-07-24)
The fine structure of acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica , was examined. This glycosaminoglycan has a major disaccharide repeating unit of -->4)-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S(1--> (where GlcNpAc is N -acetylglucosamine, IdoAp is iduronic acid, and S is sulfate)
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