生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
分子量
antigen 100 kDa
antigen 130 kDa
antigen 50 kDa
antigen 65 kDa
种属反应性
mouse, rat
技术
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 4 μg/mL using RIPA lysates of mouse M1 myeloblast
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
mouse ... Sirpa(19261)
一般描述
Signal regulatory proteins (SIRPs) are a class of transmembrane glycoproteins with extracellular domains resembling the immunoglobulins. The SIRP family consists of three members, SIRPα, SIRPβ and SIRPγ. In rat there are two SIRPβ members, SIRPβ1 and SIRPβ2. SIRP members are ubiquitously present on the surface of all immune system cells. SIRPα (SHP-1) is expressed on neurons, myeloid dendritic cells, mast cells, macrophages and granulocytes. The most studied role of SIRPα is the inhibition of host cell phagocytosis by the macrophages. SIRPα is reportedly involved in clearance of aged erythrocytes, regulation of production of TNF-α induced by LPS, controlling the migration of myeloid cells and maturation of dendritic cells
Anti-SIRPα1 (SHPS-1) recognizes the differentially glycosylated forms of SIRPα1(SHPS-1) of 130, 100 and 65 kDa. It also detects a single band at 50 kDa.
Anti-SIRPα1 (SHPS-1) recognizes the differentially glycosylated forms of SIRPα1(SHPS-1) of 130, 100 and 65 kDa. It also detects a single band at 50 kDa.
免疫原
fusion protein corresponding to the cytoplasmic domain of rat SHPS-1 (residues 418-481).
应用
Anti-SIRPα1 may be used in immunoblotting application at a working concentration of 4 μg/mL using mouse myeloblast whole cell lysate. The antibody is suitable for protein microarray and immunoprecipitation.
生化/生理作用
The antibody recognizes SIRPα1 (signal-regulatory protein) also known as BIT, SHPS-1, or p84. By immunoblotting, the antibody recognizes the differentially glycosylated forms of SIRPα1 (130, 100 and 65 kDa). It also detects a single band at 50 kDa.
外形
Solution in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
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法规信息
常规特殊物品
此项目有
Takuji Yamao et al.
The Journal of biological chemistry, 277(42), 39833-39839 (2002-08-09)
SHPS-1 is a receptor-type glycoprotein that binds and activates the protein-tyrosine phosphatases SHP-1 and SHP-2, and thereby negatively modulates intracellular signaling initiated by various cell surface receptors coupled to tyrosine kinases. SHPS-1 also regulates intercellular communication in the neural and
Ellen M van Beek et al.
Journal of immunology (Baltimore, Md. : 1950), 175(12), 7781-7787 (2005-12-13)
Signal regulatory proteins (SIRPs) constitute a family of transmembrane glycoproteins with extracellular Ig-like domains. Several SIRP family members have thus far been identified on myeloid and other cells in man, mouse, rat, and cattle. In the present study, we provide
S Adams et al.
Journal of immunology (Baltimore, Md. : 1950), 161(4), 1853-1859 (1998-08-26)
Signal-regulatory proteins (SIRP) are transmembrane glycoproteins with three extracellular Ig-like domains, closely related to Ag receptors Ig, TCR, and MHC, and a cytoplasmic domain with two immunoreceptor with tyrosine-based inhibition motifs that can interact with src homology 2 domain-containing phosphatases.
Tomomi Ishikawa-Sekigami et al.
Blood, 107(1), 341-348 (2005-09-06)
The lifespan of circulating red blood cells (RBCs) produced in bone marrow is determined by their elimination through phagocytosis by splenic macrophages. The mechanism by which RBC elimination is regulated has remained unclear, however. The surface glycoprotein SHPS-1, a member
Akiko Hayashi et al.
The Journal of biological chemistry, 279(28), 29450-29460 (2004-05-05)
SIRPbeta (signal-regulatory protein beta) is a transmembrane protein that is expressed in hematopoietic cells but whose functions are unknown. We have now cloned mouse SIRPbeta cDNA and have shown that the gene is expressed in various tissues in addition to
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