S1629
硫酸脂酶 来源于产气杆菌
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
别名:
芳基硫酸酯酶, 苯硫酸酶
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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL
type
Type VI
form
buffered aqueous glycerol solution
specific activity
2-5 units/mg protein (biuret)
mol wt
~41 kDa
concentration
10-20 units/mL
foreign activity
β-Glucuronidase ≤10 U/mL
shipped in
wet ice
storage temp.
−20°C
Quality Level
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General description
硫酸酯酶包含Cys/Ser-X-Pro-X-Arg基序，保存在它们的活性部位。
Application
产氧菌磺化酶已用于：
- 用作解凝酶处理血浆样品，通过液相色谱串联质谱（LC/MS/MS）分析进行槲皮素定量
- 基于荧光强度的酶活性检测，使用基于活性的探针1
- 处理硫硫醇脂质体，以去除硫脂中的3-O-硫代半乳糖苷头
Biochem/physiol Actions
硫酸酯酶水解硫酸酯键生成无机硫酸盐。微生物硫化酶参与硫的清除，是硫利用的必需酶。它们可能与发病机制有关。来自产气杆菌的市售硫酸盐酶可用作解凝酶，用于从缀合物中去除葡萄糖醛酸盐和硫酸盐部分。硫酸酯酶广泛用于工业和农业。
Physical form
溶于含0.01 M Tris的50%甘油，pH 7.5。
Other Notes
在pH 7.1，37℃条件下，一个单位可在每分钟水解1.0 μmole对硝基苯磷酸盐。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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