S1629
硫酸脂酶 来源于产气杆菌
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
别名:
芳基硫酸酯酶, 苯硫酸酶
一般描述
硫酸酯酶包含Cys/Ser-X-Pro-X-Arg基序，保存在它们的活性部位。
应用
产氧菌磺化酶已用于：
- 用作解凝酶处理血浆样品，通过液相色谱串联质谱（LC/MS/MS）分析进行槲皮素定量
- 基于荧光强度的酶活性检测，使用基于活性的探针1
- 处理硫硫醇脂质体，以去除硫脂中的3-O-硫代半乳糖苷头
生化/生理作用
硫酸酯酶水解硫酸酯键生成无机硫酸盐。微生物硫化酶参与硫的清除，是硫利用的必需酶。它们可能与发病机制有关。来自产气杆菌的市售硫酸盐酶可用作解凝酶，用于从缀合物中去除葡萄糖醛酸盐和硫酸盐部分。硫酸酯酶广泛用于工业和农业。
外形
溶于含0.01 M Tris的50%甘油，pH 7.5。
其他说明
在pH 7.1，37℃条件下，一个单位可在每分钟水解1.0 μmole对硝基苯磷酸盐。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)
C Gil et al.
Biochimica et biophysica acta. Biomembranes, 1861(1), 161-169 (2018-11-23)
Epsilon toxin (Etx) from Clostridium perfringens is synthesized as a very low-active prototoxin form (proEtx) that becomes active upon proteolytic activation and has the capacity to cross the blood-brain barrier (BBB), thereby producing severe neurological effects. The identity and requirements
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