表单
buffered aqueous suspension
标记范围
≥1 mg per mL
技术
affinity chromatography: suitable
基质
4% beaded agarose
基质活化
cyanogen bromide
基质附着
amino
基质隔离区
7 atoms
容量
≥15 μg/mL binding capacity (biotin)
储存温度
2-8°C
应用
来自链霉菌的链霉亲和−琼脂糖已用于：
- 在质膜转化生长因子β（TGFβ）受体II (TβRII)定量和Tβ
- RII内化过程中pull-down生物素化的细胞表面蛋白
- 生物素化miRNA pull-down实验；作为免疫沉淀的二级抗体
用于纯化含有蛋白质或DNA结合蛋白质的生物素
链亲和素琼脂糖用于蛋白质层析，亲和层析和重组蛋白表达和分析。链亲和素琼脂糖可以被用来研究烟草蚀纹病毒蛋白酶切割融合蛋白的定向固定。链亲和素琼脂糖被用来研发从天然植物提取物中筛选三重DNA结合物。
生化/生理作用
链霉亲和素是一种同型四聚体蛋白，分离自亲和素链霉菌中，与亲和素一样，对生物素具有很高的亲和力。链霉亲和素为轻度阴离子（pI ~ 5-6），非糖基化。与蛋清亲和素相比，这些特性使其非特异性结合力相对较低。链霉亲和素也比亲和素更不易被氯化胍分解成亚基。链霉亲和素-琼脂糖可用于固定或分离各种生物素化大分子和络合物（蛋白质、抗体、凝集素、核酸、受体和配体）。链霉亲和素-生物素相互作用需要苛刻的条件才能释放生物素化大分子。这一特性使得链霉亲和素-琼脂糖广泛用于各种亲和纯化应用。
外形
在pH7.2、含有0.05M NACl和0.02%叠氮化钠的0.01 M磷酸钠缓冲液中复溶
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Niusheng Xu et al.
Analytical chemistry, 84(5), 2562-2568 (2012-01-10)
A novel ligand fishing assay was established to screen triplex DNA binders from complicated samples by a combination of immobilization of triplex DNA on agarose beads and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The biotinylated oligodeoxynucleotides were first bound to
Behrad Derakhshan et al.
Nature protocols, 2(7), 1685-1691 (2007-07-21)
Covalent addition of nitric oxide (NO) to Cys-sulfur in proteins, or S-nitrosylation, plays pervasive roles in the physiological and pathophysiological modulation of mammalian protein functions. Knowledge of the specific protein Cys residues that undergo NO addition in different biological settings
I Gottschalk et al.
European journal of biochemistry, 267(23), 6875-6882 (2000-11-18)
Two cytochalasin B-binding states of the human red blood cell facilitative glucose transporter GLUT1 were studied, one exhibiting one cytochalasin B-binding site on every second GLUT1 monomer (state 1) and the other showing one site per monomer (state 2). Quantitative
Jonathan A Roberts et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(15), 4072-4082 (2007-04-13)
P2X receptors for extracellular ATP are a distinct family of ligand-gated cation channels involved in physiological processes ranging from synaptic transmission to muscle contraction. Common ATP binding motifs are absent from P2X receptors, and the extent of the agonist binding
O Litzka et al.
European journal of biochemistry, 251(3), 758-767 (1998-03-07)
In Aspergillus nidulans, a DNA-binding complex, PENR1, was shown to bind to two CCAAT-box-containing DNA elements located in the promoter regions of the bidirectionally oriented penicillin biosynthesis genes acvA and ipnA, and of the aat promoter. Here, partial purification of
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