mean particle size
20 μm
capacity
>0.15 meq/mL
storage temp.
2-8°C
Application
Ceramic HyperD media are used in protein chromatography and affinity chromatography. Ceramic HyperD media have been used to develop a chromatographic refolding process that allows processing of fusion peptides at a concentration range 10- to 100-fold higher than that observed for common refolding systems.
Features and Benefits
HyperD media are highly porous, rigid ceramic beads filled with a functionalized hydrophilic gel. The rigid bead allows extremely high linear flow rates without bed compression. The hydrogel exchanges throughout its volume, not just on the surface. And the media do not change volume due to changes in pH or ionic strength.
The supports are stable in commonly used solvents and alkaline or acid environments. Supplied as aqueous suspensions in 1 M NaCl with 20% ethanol to inhibit bacterial growth.
The supports are stable in commonly used solvents and alkaline or acid environments. Supplied as aqueous suspensions in 1 M NaCl with 20% ethanol to inhibit bacterial growth.
Legal Information
HyperD is a registered trademark of Pall Corporation
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Arne Staby et al.
Journal of chromatography. A, 1118(2), 168-179 (2006-05-09)
Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i.e. pH dependence, efficiency, binding strength, particle size distribution, static and dynamic capacity, and scanning electron microscopy (SEM) pictures. Chromatographic resins investigated were Fractogel EMD SO3- (M), Fractogel
Elisabeth Schmoeger et al.
Journal of chromatography. A, 1216(48), 8460-8469 (2009-10-27)
Refolding of proteins must be performed under very dilute conditions to overcome the competing aggregation reaction, which has a high reaction order. Refolding on a chromatography column partially prevents formation of the intermediate form prone to aggregation. A chromatographic refolding
Arne Staby et al.
Journal of chromatography. A, 1069(1), 65-77 (2005-04-23)
A comparative study was performed on heparin resins and strong and weak cation exchangers to investigate the pH dependence, efficiency, binding strength, particle size distribution, static and dynamic capacity, and scanning electron microscopy pictures of chromatographic resins. The resins tested
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