S6510
N-琥珀酰-Leu-Leu-Val-Tyr-7-氨基-4-甲基香豆素
≥90% (HPLC), Chymotrypsin substrate, powder
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关于此项目
经验公式（希尔记法）:
C40H53N5O10
CAS Number:
分子量:
763.88
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.77
Product Name
N-琥珀酰-Leu-Leu-Val-Tyr-7-氨基-4-甲基香豆素, ≥90% (HPLC)
质量水平
方案
≥90% (HPLC)
表单
powder
溶解性
0.1% trifluoroacetic acid in acetonitrile: water (3:1): 1 mg/mL, clear, colorless
储存温度
−20°C
SMILES字符串
CC(C)CC(NC(=O)CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(Cc1ccc(O)cc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2
InChI
1S/C40H53N5O10/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49)
InChI key
UVFAEQZFLBGVRM-UHFFFAOYSA-N
Amino Acid Sequence
N-Suc-Leu-Leu-Val-Tyr-7-AMC
一般描述
用作胰凝乳蛋白酶样酶的荧光底物，例如组织蛋白酶B和钙蛋白酶，其与程序性细胞死亡有关。
应用
用N-琥珀酰-亮氨酸-缬氨酸-酪氨酸-7-氨基-4-甲基香豆素测定水稻Jurkat细胞裂解液6和粗细胞裂解液中蛋白酶体类糜蛋白酶活性。7
生化/生理作用
在存在胰凝乳蛋白酶样酶活性的情况下，荧光团，7-氨基-4-甲基香豆素是从N-琥珀酰-亮氨酸-缬氨酸-酪氨酸-7-氨基-4-甲基香豆素中释放的。所获得的荧光可作为酶活性的量度。6
包装
无底玻璃瓶。内含物在插入的融合锥体内。
Izaltina Silva-Jardim et al.
Acta tropica, 91(2), 121-130 (2004-07-06)
Proteasomes are multisubunit proteases that exist universally among eukaryotes. They have multiple proteolytic activities and are believed to have important roles in regulating cell cycle, selective intracellular proteolysis, and antigen presentation. Here we have partially purified Leishmania chagasi proteasome. The
Turnover of oxidatively damaged nuclear proteins in BV-2 microglial cells is linked to their activation state by poly-ADP-ribose polymerase.
O Ullrich et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(8), 1460-1462 (2001-06-02)
T Reinheckel et al.
The Biochemical journal, 335 ( Pt 3), 637-642 (1998-10-31)
Oxidatively modified ferritin is selectively recognized and degraded by the 20S proteasome. Concentrations of hydrogen peroxide (H2O2) higher than 10 micromol/mg of protein are able to prevent proteolytic degradation. Exposure of the protease to high amounts of oxidants (H2O2, peroxynitrite
R Guerra-Sá et al.
Experimental parasitology, 109(4), 228-236 (2005-03-10)
Proteasomes are multi-subunit proteases involved in several mechanisms and thought to contribute to the regulation of cellular homeostasis. Here, we report for the first time biochemical evidence for the existence of a ubiquitin-proteasome proteolytic pathway in this parasite. Proteasomes from
C D Robertson
Molecular and biochemical parasitology, 103(1), 49-60 (1999-10-08)
As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S
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