Anti-Dimethyl-Histone H3 (diMe-Lys27) antibody, Rat monoclonal

Monoclonal Anti-dimethyl-Histone H3 (diMe-Lys27) (rat IgG2a isotype) is derived from the hybridoma K27 8H2 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a synthetic methylated peptide.

Monoclonal Anti-Dimethyl-Histone H3 (diMe-Lys27) antibody produced in rat may be used in immunoblotting and immunocytochemistry.

Methylation of histone H3 on Lys27 is associated with polycomb-mediated gene silencing in several systems. Mammals exhibit enrichment of [Me-Lys27] histone H3 at heterochromatin and [diMe-Lys27] histone H3 in euchromatin. [Me-Lys27] histone H3, also marks the inactive X chromosome. The relatively unstructured and highly charged N-terminal tail domains of histones are central to the processes that modulate chromatin structures such as acetylation, phosphorylation, and methylation, which occur particularly on Histones H3 and H4. Lysine residues can be mono-, di-, and trimethylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domain of histone H3, Lys4, Lys9 and Lys27 are the favored methylation sites.

Monoclonal Anti- dimethyl-Histone H3 (diMe-Lys27) specifically recognizes histone H3 dimethylated on Lys27 in human, bovine, mouse, rat, hamster, and Drosophila.

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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