生物来源
rat
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
6F7, monoclonal
表单
buffered aqueous solution
种属反应性
all
浓度
2-4 mg/mL
技术
western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
一般描述
单克隆抗FLAG过氧化物酶是从生长于生物反应器中的6F7杂交瘤细胞培养上清中分离出来、与辣根过氧化物酶（HRP）偶联的单克隆抗FLAG（大鼠IgG1同种型）的免疫球蛋白纯化级分。杂交瘤6F7是FLAG肽免疫大鼠的骨髓瘤细胞和脾细胞融合产生的。
该产品可识别N端、C端和内部FLAG标签融合蛋白。特别推荐用于识别C端FLAG标签融合蛋白。
免疫原
从生长于生物反应器中的6F7杂交瘤细胞培养上清中分离出来、与辣根过氧化物酶（HRP）偶联的单克隆抗FLAG（大鼠IgG1同种型）的免疫球蛋白纯化级分。
外形
溶解在0.01M磷酸缓冲盐,pH7.4, 含有0.01%硫柳汞。
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jente Stouthamer et al.
Methods in molecular biology (Clifton, N.J.), 2690, 193-204 (2023-07-14)
Interactions between extracellular domains (ECDs) are crucial for many physiological processes in the cell, most importantly perception of its environment. However, studying these often-transient interactions can be challenging. Here we describe a method that allows for in vitro detection of
Claudia Isabelle Keller Valsecchi et al.
Nature, 589(7840), 137-142 (2020-11-20)
Confinement of the X chromosome to a territory for dosage compensation is a prime example of how subnuclear compartmentalization is used to regulate transcription at the megabase scale. In Drosophila melanogaster, two sex-specific non-coding RNAs (roX1 and roX2) are transcribed
DongHyuk Lee et al.
Nature communications, 11(1), 1838-1838 (2020-04-17)
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive.
Meidi Gu et al.
Nature immunology, 22(2), 193-204 (2021-01-06)
Metabolic reprograming toward aerobic glycolysis is a pivotal mechanism shaping immune responses. Here we show that deficiency in NF-κB-inducing kinase (NIK) impairs glycolysis induction, rendering CD8+ effector T cells hypofunctional in the tumor microenvironment. Conversely, ectopic expression of NIK promotes
Jasjot Singh et al.
Nature communications, 13(1), 6212-6212 (2022-10-21)
Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in
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