SAB4200275
Anti-EIF2C2/Ago2 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~97 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
western blot: 2-4 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... EIF2C2(27161)
一般描述
The eukaryotic translation initiation factor 2C, subunit 2 (EIF2C2) or protein argonaute-2 (Ago2) belongs to the argonaute protein subfamily. The argonaute proteins contains the piwi-argonaute-zwille (PAZ), the P-element induced wimpy testis (PIWI) domain and the MID domain. Ago2 is localized to the processing bodies (P bodies) and the nucleus. The AGO2 gene is mapped to human chromosome 8q24.3.
免疫原
synthetic peptide corresponding to the N-terminal region of human EIF2C2/Ago2 conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse.
应用
Anti-EIF2C2/Ago2 (N-terminal) antibody produced in rabbit is suitable for immunoblotting.
生化/生理作用
Anti-EIF2C2/Ago2 recognizes human EIF2C2/Ago2.
The protein argonaute-2 (Ago2) is considered as a catalytic unit of mammalian RNA interference (RNAi). It possesses a slicer endonuclease activity that cleaves the target RNA in RNAi. The mRNA cleavage is possible by ribonucleoprotein complexes. Ago2 mediates tissue-specific developmental processes. Ago2 regulates both, the transcriptional and posttranslational modifications. It is implicated in the tumorigenic progression of breast cancer cell lines.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
制备说明
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Brian D Adams et al.
Endocrinology, 150(1), 14-23 (2008-09-13)
Argonaute (Ago) 2 is the catalytic engine of mammalian RNA interference, but little is known concerning the regulation of Ago2 by cell-signaling pathways. In this study we show that expression of Ago2, but not Ago1, Ago3, or Ago4, is elevated
Manal S Fawzy et al.
American journal of translational research, 12(5), 1994-2006 (2020-06-09)
MicroRNA machinery genes Argonaute 1 (AGO1) and 2 (AGO2) are associated with several hallmarks of cancer. They play a key role in transcriptomic silencing, regulation of the immune system, cell differentiation, and angiogenesis processes. The present pilot study aims to
Jidong Liu et al.
Science (New York, N.Y.), 305(5689), 1437-1441 (2004-07-31)
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute family proteins. Here, we show that the multiple Argonaute proteins present in mammals are
Lasse Peters et al.
Molecular cell, 26(5), 611-623 (2007-06-15)
Small regulatory RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi interacting RNAs (piRNAs) have been discovered in the past, and it is becoming more and more apparent that these small molecules have key regulatory functions. Small RNAs
Gunter Meister et al.
Molecular cell, 15(2), 185-197 (2004-07-21)
Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To
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