SAB4200289
Anti-Dystrobrevin-β (N-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
别名:
Anti-DTNB
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~72 kDa
species reactivity
rat, human
concentration
~1.5 mg/mL
technique(s)
immunohistochemistry: 20-40 μg/mL using formalin-fixed, paraffin-embedded rat kidney., indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells overexpressing human dystrobrevin-β., western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human dystrobrevin-β.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DTNB(1838)
rat ... Dtnb(362715)
General description
The dystrobrevin-β(DTNB) gene is mapped to human chromosome 2p23.3. The encoded protein belongs to the dystrobrevin (DB) subfamily of the dystrophin-related protein family. DB is a component of the dystrophin-glycoprotein complex (DGC) that is located at the muscle sarcolemma and forms a transmembrane link between the cytoskeleton and the basal lamina. β-DB is confined to non-muscle tissues and predominantly expressed in the kidney and brain. β-DB is linked to dystrophin and syntrophin isoforms in the hippocampus, cortex, and Purkinje cells. It is abundant in the post-synaptic densities (PSDs).
Immunogen
synthetic peptide corresponding to a sequence located at the N-terminus of human dystrobrevin-b (DTNB), conjugated to KLH. The corresponding sequence is identical in human DTNB isoforms 1-5, and highly conserved (single amino acid substitution) in rat and mouse DTNB.
Application
Anti-Dystrobrevin-β (N-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Anti-Dystrobrevin-b(N-terminal) specifically recognizes human and rat dystrobrevin-b.
Dystrobrevin-β (β-DB) may be significantly involved in assembling and supporting the dystrophin-associated protein complex (DPC)-like complexes in non-muscle tissues. It is known to interact with the neuronal kinesin heavy chain Kif5A and Kif5B in the brain, suggesting that β-DB might act as a motor protein receptor involved in the transport of dystrophin-glycoprotein complex (DGC) components to specific sites in the cell.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
P Macioce et al.
Journal of cell science, 116(Pt 23), 4847-4856 (2003-11-06)
Beta-dystrobrevin, a member of the dystrobrevin protein family, is a dystrophin-related and -associated protein restricted to non-muscle tissues and is highly expressed in kidney, liver and brain. Dystrobrevins are now thought to play an important role in intracellular signal transduction
Maria Santa Rocca et al.
European journal of medical genetics, 56(1), 62-65 (2012-11-13)
Deletions of the short arm of chromosome 2 are exceedingly rare and only nine cases involving regions from 2p23 to 2pter have been reported to date. Most of these deletions had only been analysed by GTG banding. Here, we report
T Haenggi et al.
Cellular and molecular life sciences : CMLS, 63(14), 1614-1631 (2006-05-20)
The dystrophin glycoprotein complex (DGC) is a multimeric protein assembly associated with either the X-linked cytoskeletal protein dystrophin or its autosomal homologue utrophin. In striated muscle cells, the DGC links the extracellular matrix to the actin cytoskeleton and mediates three
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