SAB4200360
Anti-Cyclophilin B antibody, Mouse monoclonal
clone CYPB-3, purified from hybridoma cell culture
别名:
Monoclonal Anti-CYP-S1, Monoclonal Anti-CYPB, Monoclonal Anti-OI9, Monoclonal Anti-PPIB, Monoclonal Anti-SCYLP, Monoclonal Anti-peptidylprolyl isomerase B
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CYPB-3, monoclonal
Application:
western blot
Species reactivity:
human, mouse, rat
Citations:
8
Technique(s):
western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.
Uniprot accession no.:
产品名称
Anti-Cyclophilin B antibody, Mouse monoclonal, clone CYPB-3, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CYPB-3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~20 kDa
species reactivity
human, mouse, rat
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PPIB(5479)
mouse ... Ppib(19035)
rat ... Ppib(64367)
Application
Monoclonal Anti-Cyclophilin B antibody produced in mouse has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Cyclophilin B (CypB) is involved in inflammation, viral infection and cancer. It also plays an important role in protecting cells against endoplasmic reticulum (ER) stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.
In endoplasmic reticulum (ER), Cyclophilin B works as a chaperone for exported proteins. It also participates in the intracellular transport of collagen and proper formation of collagen fibril. Cyclophilin B can bind to the Gag protein of human immunodeficiency type 1 (HIV-1) and helps in HIV replication. It is also involved in the infection of hepatitis C virus (HCV), Japanese encephalitis virus (JEV) and human papillomavirus type 16 (HPV16). Cyclophilin B protects against ER-mediated hypoxia-induced cell death. It is overexpressed in various malignancies.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cyclophilin B belongs to the cyclophilin family of immunophilins. It is present in the cytoplasm, nucleus, endoplasmic reticulum (ER)/golgi and plasma membrane. The protein can also be secreted from cells. The associated gene is mapped to human chromosome 15q22.
Monoclonal Anti-Cyclophilin B (mouse IgG1 isotype) is derived from the hybridoma CYPB-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to keyhole limpet hemocyanin (KLH). Cyclophilin B (CypB) is a 21 kDa protein encoded by the peptidylprolyl isomerase B (PPIB) gene that belongs to the cyclophilin family, is a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types.
Immunogen
synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to KLH. The corresponding sequence is identical in mouse, monkey and canine and differs by one amino acid in rat Cyclophilin B.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Chapter 12 - Radiology
Adams JE, et al.
Pediatric Bone (2012)
Hsp47 and Cyclophilin B Traverse the Endoplasmic Reticulum with Procollagen into Pre-Golgi Intermediate Vesicles A ROLE FOR Hsp47 AND CYCLOPHILIN B IN THE EXPORT OF PROCOLLAGEN FROM THE ENDOPLASMIC RETICULUM
Smith T, et al.
The Journal of Biological Chemistry, 270(31) (1995)
Cyclophilin B is involved in p300-mediated degradation of CHOP in tumor cell adaptation to hypoxia.
Jeong K, et al.
Cell Death and Differentiation, 21, 438-438 (2014)
Cyclophilin B supports Myc and mutant p53-dependent survival of glioblastoma multiforme cells.
Choi JW, et al.
Cancer Research, 74, 484-484 (2014)
What is new in genetics and osteogenesis imperfecta classification?
Valadares ER, et al.
Jornal de Pediatria, 90, 536-536 (2014)
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持