SAB4200413
Monoclonal Anti-LIN28 antibody produced in mouse
clone L28.5, tissue culture supernatant
别名:
Monoclonal Anti-CSDD1, Monoclonal Anti-LIN28A, Monoclonal Anti-ZCCHC1
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352202
Conjugate:
unconjugated
Clone:
L28.5, monoclonal
Application:
WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
tissue culture supernatant
antibody product type
primary antibodies
clone
L28.5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~28 kDa
species reactivity
mouse, human
technique(s)
western blot: 1:8,000-1:16,000 using extracts of Ntera-2 cells.
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LIN28A(79727)
mouse ... Lin28a(83557)
General description
LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Application
Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended.
Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A.
Biochem/physiol Actions
The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.
Physical form
Solution containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Florian Mayr et al.
International journal of molecular sciences, 14(8), 16532-16553 (2013-08-14)
Lin28 is an essential RNA-binding protein that is ubiquitously expressed in embryonic stem cells. Its physiological function has been linked to the regulation of differentiation, development, and oncogenesis as well as glucose metabolism. Lin28 mediates these pleiotropic functions by inhibiting
Linbo Wang et al.
PloS one, 8(6), e67373-e67373 (2013-07-11)
Resistance to radiation therapy is a major obstacle for the effective treatment of cancers. Lin28 has been shown to contribute to breast tumorigenesis; however, the relationship between Lin28 and radioresistance remains unknown. In this study, we investigated the association of
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