SAB4200490
Anti-DLC1 (C-terminal region) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-Deleted in Liver Cancer 1, ARHGAP7, Anti-HP, Anti-STARD12, Anti-p122-RhoGAP
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, IP, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~200 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon., immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells overexpressing human DLC1., indirect immunofluorescence: 1-2 μg/mL using MDBK cells., western blot: 1.5-3 μg/mL using lysates of HEK-293T cells overexpressing human DLC1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DLC1(10395)
General description
Deleted in liver cancer 1 (DLC1), also known as ARHGAP7, is a candidate tumor suppressor gene, originally isolated from human hepatocellular carcinoma (HCC). DLC1 is mapped to chromosome 8p21.3-22 and is also expressed in normal human tissues. DLC1 protein comprises three domains namely steroidogenic acute regulatory related lipid transfer (START) domain, sterile a-motif (SAM) and Rho GTPase-activating protein (RhoGAP) domain.
Immunogen
synthetic peptide corresponding to a sequence near the C-terminal of human DLC1 isoform 1, conjugated to KLH.
Application
Anti-DLC1 (C-terminal region) antibody produced in rabbit may be used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Anti-DLC1(C-terminal region) specifically recognizes human DLC1.
Deleted in liver cancer 1 (DLC1) interacts with focal adhesion protein, tensin 2. DLC1 gene locus is recurrently deleted in solid tumors as well as in hepatocellular carcinoma (HCC). The Rho GTPase-activating protein (RhoGAP) region is responsible for the growth inhibitory effect in HCC. It has been shown that the N-terminal region of DLC1 localizes to focal adhesions by binding to SH2 domains on tensins. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The cell motility and morphology maintenance functionality of DLC1 is primarily mediated by steroidogenic acute regulatory protein (STAR)- GTPase-activating proteins (GAP1) region.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Katsuhisa Kawai et al.
Genes to cells : devoted to molecular & cellular mechanisms, 14(2), 227-241 (2009-01-28)
There is a class of GTPase activating proteins for the Rho family GTPases (RhoGAPs) that contain the steroidogenic acute regulatory protein (STAR)-related lipid transfer (START) domain. In mammals three genes encode such proteins and they are designated START-GAP1-3 or deleted
Wen Xue et al.
Genes & development, 22(11), 1439-1444 (2008-06-04)
Deletions on chromosome 8p are common in human tumors, suggesting that one or more tumor suppressor genes reside in this region. Deleted in Liver Cancer 1 (DLC1) encodes a Rho-GTPase activating protein and is a candidate 8p tumor suppressor. We
Yi-Chun Liao et al.
Cancer research, 68(19), 7718-7722 (2008-10-03)
Deleted in liver cancer-1 (DLC-1) is a RhoGTPase-activating protein (RhoGAP) domain containing tumor suppressor that is often down-regulated in various cancer types. Previously, we have shown that DLC-1 is recruited to focal adhesions by binding to the Src homology 2
Judy Wai Ping Yam et al.
Cancer research, 66(17), 8367-8372 (2006-09-05)
Deleted in liver cancer 1 (DLC1) is a recently identified tumor suppressor gene frequently underexpressed in hepatocellular carcinoma (HCC). DLC1 encodes a Rho GTPase-activating protein domain that exhibits growth-suppressive activity in HCC cell lines. Our recent finding has revealed that
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