Anti-DIS3 antibody, Mouse monoclonal

Monoclonal Anti-DIS3 (mouse IgG1 isotype) is derived from the hybridoma DIS3-N produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3), mitotic control homolog, also known as RRP44 and EXOSC11, is a catalytic component of the eukaryotic RNA exosome. The RNA exosome is a ribonucleolytic complex of 400 kDa. It is composed of a nine catalytically inert subunit core. The exosome is a nuclear complex, also localized to the cytoplasm. DIS3 is predominantly found in the nucleus, whereas DIS3L is mainly cytoplasmic.

Synthetic peptide corresponding to an internal sequence of human DIS3, conjugated to KLH. The corresponding sequence is identical in monkey, pig and dog, and differs by a single amino acid in mouse and rat DIS3.

Monoclonal Anti-DIS3 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

The RNA exosome is essential for RNA processing and turnover. Its core subunits possess structural function and is associated with substrate recognition. The exosome was characterized as a multiunit complex important for the 3′ → 5′ processing and degradation of many types of RNAs including mRNA, rRNA, snRNA, snoRNA, and tRNA. DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3) and DIS3L are potential exonucleases. DIS3 also displays endonuclease activity.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.