SAB4200660
Monoclonal Anti-GOLPH3 antibody produced in mouse
clone GOL3-1, purified from hybridoma cell culture
别名:
GOPP1, GPP34, MIDAS, golgi phosphoprotein 3 (coat-protein)
产品名称
Monoclonal Anti-GOLPH3 antibody produced in mouse, clone GOL3-1, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GOL3-1
form
buffered aqueous solution
mol wt
antigen ~34 kDa
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
immunoblotting: 2.5-5 μg/mL using using whole extracts of rat PC-12 cells.
immunofluorescence: 2.5-5 μg/mL using mouse 3T3 cells
immunoprecipitation (IP): 5-10 μg using using lysates of human HeLa cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)
Application
Monoclonal Anti-GOLPH3 antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
GOLPH3 (golgi phosphoprotein 3) links Golgi membrane to actin cytoskeleton by interacting with MYO18A, which is an unconventional myosin. This, in turn, is crucial for Golgi architecture and vesicle trafficking. It is an oncogene, which is susceptible to amplification in cancers, and is responsible for controlling the response to the anti-cancer drug, rapamycin. It is thought to function as a coat protein during Golgi trafficking.
GOLPH3 has a role in Golgi trafficking and morphology. It interacts with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. GOLPH3 is also required for Golgi function. Overexpression of GOLPH3 is correlated with hyperactivation of mTOR signaling, in human cancer cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
GOLPH3 (golgi phosphoprotein 3) is an abundant phosphatidylinositol-4-phosphate (PtdIns4P)-interacting protein which is conserved from yeast to humans. This protein is localized to Golgi through PtdIns4P. This gene is localized to human chromosome 5p13. GOLPH3 has a molecular weight of 34kDa.
Monoclonal Anti-GOLPH3 (mouse IgG1 isotype) is derived from the hybridoma GOL3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. GOLPH3 (Golgi phosphoprotein 3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. GOLPH3 is conserved from yeast to human. This gene was described as an oncogene that is commonly amplified in human cancer and in cancer cell lines.
Immunogen
synthetic peptide corresponding to a sequence at the N-terminal region of human Golph3
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Holly C Dippold et al.
Cell, 139(2), 337-351 (2009-10-20)
Golgi membranes, from yeast to humans, are uniquely enriched in phosphatidylinositol-4-phosphate (PtdIns(4)P), although the role of this lipid remains poorly understood. Using a proteomic lipid-binding screen, we identify the Golgi protein GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p)
A W Bell et al.
The Journal of biological chemistry, 276(7), 5152-5165 (2000-10-24)
A mass spectrometric analysis of proteins partitioning into Triton X-114 from purified hepatic Golgi apparatus (84% purity by morphometry, 122-fold enrichment over the homogenate for the Golgi marker galactosyl transferase) led to the unambiguous identification of 81 proteins including a
Kenneth L Scott et al.
Nature, 459(7250), 1085-1090 (2009-06-26)
Genome-wide copy number analyses of human cancers identified a frequent 5p13 amplification in several solid tumour types, including lung (56%), ovarian (38%), breast (32%), prostate (37%) and melanoma (32%). Here, using integrative analysis of a genomic profile of the region
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