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Merck
CN

SHC007

MISSION® pLKO.1-puro 荧光素酶 shRNA 对照质粒 DNA

shRNA sequence targeting luciferase

别名:

MISSION® 对照载体

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关于此项目

UNSPSC Code:
41106609
NACRES:
NA.51
MDL number:
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
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product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Quality Level

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General description

MISSION ® 荧光素酶 shRNA 对照载体是一个 7,091 碱基对的慢病毒质粒载体,含有靶向来自 Photinus pyralis 荧光素酶的 shRNA 序列(GenBank 登记号:M15077)。荧光素酶 shRNA 对照载体在使用表达萤火虫荧光素酶的细胞系的实验中作为阳性敲除对照十分有用。其也可作为阴性对照载体。

氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外,通过与相容的包装质粒共转染,可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒,MISSION ® 慢病毒包装混合物(产品编号:SHP001)。荧光素酶 shRNA 对照载体以 10μg 质粒 DNA,溶于 Tris-EDTA (TE) 缓冲液中,浓度为 500 ng/μl.

Application

想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

Legal Information

使用本产品需遵守一个或多个许可协议。有关详细信息,请参阅http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

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Islands of spatially discordant APD alternans underlie arrhythmogenesis by promoting electrotonic dyssynchrony in models of fibrotic rat ventricular myocardium.
Majumder R
Scientific Reports, 6 (2016)
Deepak Bararia et al.
Nature communications, 7, 10968-10968 (2016-03-24)
CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor for myeloid lineage commitment. Here we demonstrate that acetylation of C/EBPα at lysine residues K298 and K302, mediated at least in part by general control non-derepressible 5 (GCN5), impairs C/EBPα DNA-binding
Ivana Horvathova et al.
Molecular cell, 68(3), 615-625 (2017-10-24)
RNA degradation plays a fundamental role in regulating gene expression. In order to characterize the spatiotemporal dynamics of RNA turnover in single cells, we developed a fluorescent biosensor based on dual-color, single-molecule RNA imaging that allows intact transcripts to be
Vajiheh Neshati et al.
Applied biochemistry and biotechnology, 186(1), 245-255 (2018-03-27)
Since the adult mammalian heart has limited regenerative capacity, cardiac trauma, disease, and aging cause permanent loss of contractile tissue. This has fueled the development of stem cell-based strategies to provide the damaged heart with new cardiomyocytes. Bone marrow-derived mesenchymal
The epigenetic regulators CBP and p300 facilitate leukemogenesis and represent therapeutic targets in acute myeloid leukemia.
Giotopoulos G
Oncogene, 35(3), 279-289 (2016)

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