SML3992
Rhod-5N potassium salt
≥95% (HPLC)
别名:
9-[4-[bis(Carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-nitrophenoxy]ethoxy]phenyl]-3,6-bis(dimethylamino)-xanthylium, potassium salt, Rhod 5N potassium salt, Rhod5N potassium salt, Rhodamine-5N potassium salt
质量水平
方案
≥95% (HPLC)
表单
powder
储存条件
desiccated
颜色
, faint red to black
溶解性
H2O: 2 mg/mL, clear
储存温度
-10 to -25°C
SMILES字符串
O=C(O)CN(C1=C(C=C(C=C1)C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCCOC5=CC([N+]([O-])=O)=CC=C5N(CC(O)=O)CC([O-])=O)CC(O)=O.[K]
生化/生理作用
Low-affinity fluorescent Ca2+ probe for measuring relatively high [Ca2+].
Rhod-5N is a low-affinity fluorescent Ca2+ probe (Kd = 320 μM) that is preferred over high-affinity probes, such as Rhod-2 (Kd = 570 nM), for measuring [Ca2+] when very little buffering can be tolerated or in subcellular compartments where relatively high Ca2+ levels are expected, such as mitochondria (MT) and endoplasmic reticulum (ER). Ex/Em = 551/576 nm.
Rhod-5N is a low-affinity fluorescent Ca2+ probe (Kd = 320 μM) that is preferred over high-affinity probes, such as Rhod-2 (Kd = 570 nM), for measuring [Ca2+] when very little buffering can be tolerated or in subcellular compartments where relatively high Ca2+ levels are expected, such as mitochondria (MT) and endoplasmic reticulum (ER). Ex/Em = 551/576 nm.
免责声明
Hygroscopic
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Gavriel David et al.
Cell calcium, 33(3), 197-206 (2003-02-26)
Peak values reported for mitochondrial matrix [Ca(2+)] following stimulation have ranged from micromolar to near-millimolar in various cells. Measurements using fluorescent indicators have traditionally used high-affinity dyes such as rhod-2, whose fluorescence would be expected to saturate if matrix [Ca(2+)]
Stephen Hollingworth et al.
Biophysical journal, 97(7), 1864-1872 (2009-10-07)
The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of
Mathieu Soibinet et al.
Journal of fluorescence, 18(6), 1077-1082 (2008-05-20)
The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of
Sergio de la Fuente et al.
Cell calcium, 51(1), 65-71 (2011-12-03)
Available methods to measure mitochondrial [Ca(2+)] ([Ca(2+)](M)) include both targeted proteins and fluorescent dyes. Targeted proteins usually report much higher [Ca(2+)](M) values than fluorescent dyes, up to two orders of magnitude. However, we show here that the low-Ca(2+)-affinity dye rhod-5N
R Madelaine Paredes et al.
Methods (San Diego, Calif.), 46(3), 143-151 (2008-10-22)
Our understanding of the underlying mechanisms of Ca2+ signaling as well as our appreciation for its ubiquitous role in cellular processes has been rapidly advanced, in large part, due to the development of fluorescent Ca2+ indicators. In this chapter, we
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