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Merck
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SRE0005

蛋白酶 K 来源于林伯氏白色念球菌

Reagents designed and manufactured under current ISO 13485 certification.

别名:

肽链内切酶

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关于此项目

化学文摘社编号:
39450-01-6
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
254-457-8
MDL number:
MFCD00132129
EC 号:
3.4.21.64 (BRENDA, IUBMB)

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产品名称

蛋白酶 K 来源于林伯氏白色念球菌, Reagents designed and manufactured under current ISO 13485 certification.

biological source

microbial

grade

Molecular Biology

form

buffered aqueous glycerol solution

mol wt

28.93 kDa

storage condition

dry at room temperature

concentration

≥10 mg/mL protein
≥800 unit/mL

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤500 pg/mg DNA (PicoGreen® assay)

color

almost colorless

pH

7.5

suitability

suitable for gel electrophoresis and DNA isolation procedures

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

shipped in

wet ice

storage temp.

2-8°C

Quality Level

500

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Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Other Notes

在pH 7.5，37 °C 条件下，一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。

Legal Information

PicoGreen is a registered trademark of Life Technologies

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000426414
0000426414
0000316051
0000316049
0000316053

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