SRP0167
DNMT3B Active from mouse
recombinant, expressed in baculovirus infected insect cells, ≥30% (SDS-PAGE)
别名:
DNA (cytosine-5-)-methyltransferase 3b, DNA MTase MmuIIIB
生物来源
mouse
重组
expressed in baculovirus infected insect cells
方案
≥30% (SDS-PAGE)
表单
aqueous solution
分子量
99 kDa
包装
pkg of 10 μg
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
mouse ... DNMT3B(13436)
一般描述
Mouse DNMT3B (DNA methyltransferase 3 beta) belongs to the murine Dnmt3 family. It is expressed at high levels in undifferentiated ES cells. Gene is located on chromosome 20 ICF locus. DNMT3B codes for a functional DNA methyltransferase. In human, DNMT3B gene is mapped to chromosome 20q11.2. In mouse, Dnmt3b gene is located on the chromosome 20 ICF locus.
应用
Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
生化/生理作用
DNMT3B (DNA methyltransferase 3 beta) controls DNA methylation. Mutations in DNMT3B results in ICF syndrome (immunodeficiency, centromeric instability, facial anomalies). Dnmt3b is required for de novo methylation and mammalian development. It is also essential for methylation of centromeric minor satellite repeats.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome.
Hansen RS, et al.
Proceedings of the National Academy of Sciences of the USA, 96(25), 14412-14417 (1999)
Increased DNA methyltransferase 3b (Dnmt3b)-mediated CpG island methylation stimulated by oxidative stress inhibits expression of a gene required for neural tube and neural crest development in diabetic pregnancy.
Wei D & Loeken MR
Diabetes, 63(10), 3512-3522 (2014)
DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.
Okano M, et al.
Cell, 99(3), 247-257 (1999)
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