SRP3237
TRAIL from mouse
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
别名:
Apo2 Ligand, TL2, TNF-related apoptosis-inducing Ligand, TNFSF10
生物来源
mouse
重组
expressed in E. coli
方案
≥95% (HPLC)
≥95% (SDS-PAGE)
表单
lyophilized
效能
40.0-60.0 ng/mL ED50
分子量
20.0 kDa
包装
pkg of 50 μg
技术
cell culture | mammalian: suitable
杂质
<0.1 EU/μg endotoxin, tested
颜色
white
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
mouse ... TNFSF10(22035)
一般描述
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a cytotoxic protein. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.
生化/生理作用
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis is achieved through binding to two death-signaling receptors, death receptor 4 and 5 (DR4 and 5). These receptors belong to the tumor necrosis factor receptor (TNFR) superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell′s apoptotic machinery.
外形
Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
制备说明
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
其他说明
MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
S A Marsters et al.
Recent progress in hormone research, 54, 225-234 (1999-11-05)
Apo2 ligand (Apo2L, also called TRAIL) is a member of the tumor necrosis factor (TNF) cytokine family. The closest homolog of Apo2L is CD95 (Fas/Apo1) ligand, to which it has 24% amino acid sequence identity. Similar to CD95L, Apo2L activates
Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway.
Chaudhary PM
Immunity, 7(6), 821-830 (1997)
Apoptosis by death factor.
S Nagata
Cell, 88(3), 355-365 (1997-02-07)
Abdul G Hameed et al.
The Journal of experimental medicine, 209(11), 1919-1935 (2012-10-17)
Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by the progressive narrowing and occlusion of small pulmonary arteries. Current therapies fail to fully reverse this vascular remodeling. Identifying key pathways in disease pathogenesis is therefore required for the development
W Zeng et al.
Cell death & disease, 5, e1282-e1282 (2014-06-06)
Nascent polypeptide-associated complex α (αNAC) is reportedly overexpressed in several types of cancers and regulates cell apoptosis under hypoxic conditions in HeLa cells. The aim of our study was to investigate the apoptotic function of αNAC in cancer progression. First
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