Thrombospondin human

The gene TSP1 (thrombospondin 1) is mapped to human chromosome 15q14. It is a matricellular glycoprotein and can be secreted by many cells. Recombinant human thrombospondin is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Human thrombospondin is purified by proprietary chromatographic techniques.

In rat C6 astroglioma cells, TSP1 (thrombospondin 1)-human has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression. It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.

TSP1 (thrombospondin 1) is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. TSP1 expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP1 stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α) inhibits TSP1 production in these cells. In endothelial cells, it controls adhesion, migration as well as proliferation. It also exhibits antiangiogenic properties and regulates immune processes. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. It also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. TSP1 has been used as a potential regulator of tumor growth and metastasis. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis. Variants of this gene might be linked with increased risk of autism.

Reconstitute with water at the concentration 0.1 mg/ml. Avoid freeze-thaw cycles.